5-Fluoroorotic acid as a selective agent in yeast molecular genetics

Methods Enzymol. 1987;154:164-75. doi: 10.1016/0076-6879(87)54076-9.

Abstract

5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Genes, Fungal
  • Genetic Engineering / methods
  • Mutation*
  • Orotic Acid / analogs & derivatives*
  • Orotic Acid / pharmacology
  • Plasmids
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development

Substances

  • Orotic Acid
  • 5-fluoroorotic acid