Abstract
5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Genes, Fungal
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Genetic Engineering / methods
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Mutation*
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Orotic Acid / analogs & derivatives*
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Orotic Acid / pharmacology
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Plasmids
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
Substances
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Orotic Acid
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5-fluoroorotic acid