Identification of components of a new stability system of plasmid R1, ParD, that is close to the origin of replication of this plasmid

Mol Gen Genet. 1987 Nov;210(1):101-10. doi: 10.1007/BF00337764.


We provide evidence that a mutation which derepresses an autoregulated system that is located in the vicinity of the basic replicon of R1, stabilizes the ParA- and ParB- miniplasmid of R1 pKN1562, without increasing its copy number. The system, which we have called ParD, maps inside the 1.45-kb PstI-EcoRI fragment that is adjacent to the origin of replication of the plasmid. Two proteins whose expression is coordinated are components of the system. The sequence of the PstI-EcoRI fragment was obtained. The wild-type ParD system determines in cis a basal but detectable stability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Sequence
  • Chromosome Mapping
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics*
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Mutation
  • R Factors*
  • Replicon*


  • Bacterial Proteins
  • DNA, Bacterial

Associated data

  • GENBANK/X06240