Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Nov 2:28:e00553.
doi: 10.1016/j.btre.2020.e00553. eCollection 2020 Dec.

Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67

Zhaobo Wang  1   2 , Dajun Ren  1   2 , Hongyan Yu  1   2 , Shan Jiang  1   2 , Shuqin Zhang  1   2 , Xiaoqing Zhang  1   2
Affiliations

Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67

Zhaobo Wang et al. Biotechnol Rep (Amst). .

Abstract

Laccase (Trametes versicolor) was immobilized onto/into zeolite imidazolate framework-67 (ZIF-67) for the first time, materials were performed by assisting one-pot synthesis strategy in aqueous solution at room temperature. The resulting laccase@ZIF-67 composite was characterized by powder X-ray diffraction (PXRD), fourier transform infrared (FT-IR) spectroscopies, field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray (EDX), thermal gravimetric analyses (TGA). The results showed that laccase could be well immobilized on ZIF-67 materials. Laccase@ZIF-67 was stable on storage stability and reuseablility, retaining 88 % (15 days at 4 °C) and 59 % (five reaction cycles) of residual enzyme activity. Laccase@ZIF-67 exhibited relatively stable activity at pH 3-5. In addition, the thermal deactivation kinetic studies of laccase@ZIF-67 showed a lower k value, higher t1/2 and ΔG values along with the enhancement of thermodynamic parameters than that of free laccase, and laccase@ZIF-67 had excellent level of thermostability.

Keywords: Enzyme activity; Enzyme immobilization; Laccase; Stability; Zeolite imidazolate framework-67 (ZIF-67).

PubMed Disclaimer

Conflict of interest statement

The authors report no declarations of interest.

Figures

None
Graphical abstract
Scheme 1
Scheme 1
Schematic representation and photograph of the synthesis of the pure ZIF-67, immobilized laccase (laccase@ZIF-67) composite and reused immobilized laccase (RIL@ZIF-67).
Scheme 2
Scheme 2
Mechanism of catalytic oxidation of ABTS.
Fig. 1
Fig. 1
PXRD patterns of pure ZIF-67, laccas@ZIF-67 and RIL@ZIF-67.
Fig. 2
Fig. 2
FT-IR spectra of free laccase, pure ZIF-67, laccase@ZIF-67 and RIL@ZIF-67.
Fig. 3
Fig. 3
FE-SEM images of (a) ZIF-67, (b) laccase@ZIF-67 and (c) RIL@ZIF-67. (d) Energy dispersive X-ray spectrum of ZIF-67 and laccase@ZIF-67.
Fig. 4
Fig. 4
TGA curves of pure laccase (black), ZIF-67 (red) and laccase@ZIF-67 (blue) (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).
Fig. 5
Fig. 5
Storage stability of free laccase and laccase@ZIF-67.
Fig. 6
Fig. 6
Reusability of laccase@ZIF-67 up to 5th reuse cycle.
Fig. 7
Fig. 7
(a) The relative enzyme activity of free laccase and laccase@ZIF-67. (b) The deactivation curve of free laccase and laccase@ZIF-67.
Fig. 8
Fig. 8
(a) The relative enzyme activity of free laccase and laccase@ZIF-67. (b) Thermal deactivation kinetic studies of free laccase and laccase@ZIF-67.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Haritash A.K., Kaushik C.P. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. J. Hazard. Mater. 2009;169(1-3):1–15. - PubMed
    1. Sousa A.C., Conceicao Oliveira M., Martins L.O., Robalo M.P. A sustainable synthesis of asymmetric phenazines and phenoxazinones mediated by CotA‐Laccase. Adv. Synth. Catal. 2018;360(3):575–583.
    1. Mohammadi M., As’habi M.A., Salehi P., Yousefi M., Nazari M., Brask J. Immobilization of laccase on epoxy-functionalized silica and its application in biodegradation of phenolic compounds. Int. J. Biol. Macromol. 2018;109:443–447. - PubMed
    1. Barrios-Estrada C., de Jesús Rostro-Alanis M., Muñoz-Gutiérrez B.D., Iqbal H.M., Kannan S., Parra-Saldívar R. Emergent contaminants: endocrine disruptors and their laccase-assisted degradation–a review. Sci. Total Environ. 2018;612:1516–1531. - PubMed
    1. Yun N., He B. Effects of enzyme treatment on surface properties of Mg (OH) 2-based peroxide bleached deinked pulp. BioResources. 2019;14(2):2861–2872.

LinkOut - more resources