An inducible CRISPR interference library for genetic interrogation of Saccharomyces cerevisiae biology

Commun Biol. 2020 Nov 27;3(1):723. doi: 10.1038/s42003-020-01452-9.


Genome-scale CRISPR interference (CRISPRi) is widely utilized to study cellular processes in a variety of organisms. Despite the dominance of Saccharomyces cerevisiae as a model eukaryote, an inducible genome-wide CRISPRi library in yeast has not yet been presented. Here, we present a genome-wide, inducible CRISPRi library, based on spacer design rules optimized for S. cerevisiae. We have validated this library for genome-wide interrogation of gene function across a variety of applications, including accurate discovery of haploinsufficient genes and identification of enzymatic and regulatory genes involved in adenine and arginine biosynthesis. The comprehensive nature of the library also revealed refined spacer design parameters for transcriptional repression, including location, nucleosome occupancy and nucleotide features. CRISPRi screens using this library can identify genes and pathways with high precision and a low false discovery rate across a variety of experimental conditions, enabling rapid and reliable assessment of genetic function and interactions in S. cerevisiae.

Publication types

  • Research Support, N.I.H., Extramural
  • Validation Study

MeSH terms

  • Adenine / biosynthesis
  • Arginine / biosynthesis
  • CRISPR-Cas Systems*
  • Gene Library*
  • Genes, Fungal
  • Plasmids
  • Saccharomyces cerevisiae / genetics*


  • Arginine
  • Adenine