Solimonas marina sp. nov., isolated from deep seawater of the Pacific Ocean

Abstract

A taxonomic study was carried out on strain C16B3^T, which was isolated from deep seawater of the Pacific Ocean. The bacterium was Gram-stain-negative, oxidase- and catalase- positive and rod-shaped. Growth was observed at salinities of 0-8.0 % and at temperatures of 10-45 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C16B3^T belonged to the genus Solimonas, with the highest sequence similarity to Solimonas terrae KIS83-12^T (97.2 %), followed by Solimonas variicoloris MN28^T (97.0 %) and the other four species of the genus Solimonas (94.5 -96.8 %). The average nucleotide identity and estimated DNA-DNA hybridization values between strain C16B3^T and the type strains of the genus Solimonas were 74.05-79.48 % and 19.5-22.5 %, respectively. The principal fatty acids (>5 %) were summed feature 8 (C_{18 : 1} ω7c/C_{18 : 1} ω6c; 20.9 %), iso-C_{16 : 0} (14.6 %), C_{16 : 1} ω5c (9.4 %), iso-C_{12 : 0} (8.4 %), summed feature 2 (C_{14 : 0} 3-OH/iso I-C_{16 : 1} and C_{12 : 0} aldehyde; 6.8 %) and C_{16 : 0} (5.5 %). The G+C content of the chromosomal DNA was 65.37 mol%. The respiratory quinone was determined to be Q-8 (100 %). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified aminolipids, six unidentified phospholipids and one unidentified polar lipid. The combined genotypic and phenotypic data show that strain C16B3^T represents a novel species within the genus Solimonas, for which the name Solimonas marina sp. nov. is proposed, with the type strain C16B3^T (=MCCC 1A04678^T=KCTC 52314^T).

Keywords: Pacific Ocean; Solimonas marina sp. nov.; deep seawater; taxonomy.