ELISA detection of SARS-CoV-2 antibodies in saliva

Sci Rep. 2020 Nov 30;10(1):20818. doi: 10.1038/s41598-020-77555-4.


To facilitate containment of the COVID-19 pandemic currently active in the United States and across the world, options for easy, non-invasive antibody testing are required. Here we have adapted a commercially available, serum-based enzyme-linked immunosorbent assay (ELISA) for use with saliva samples, achieving 84.2% sensitivity and 100% specificity in a set of 149 clinical samples. This strategy will enable widespread, affordable testing for patients who experienced this disease, whilst minimizing exposure risk for healthcare workers.

MeSH terms

  • Antibodies, Viral / analysis*
  • COVID-19 / diagnosis*
  • COVID-19 Serological Testing / methods*
  • Carrier State / diagnosis
  • Clinical Laboratory Techniques
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Immunoglobulin A / analysis
  • Immunoglobulin G / analysis
  • Immunoglobulin M / analysis
  • SARS-CoV-2 / immunology
  • Saliva / immunology*
  • Sensitivity and Specificity


  • Antibodies, Viral
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M