Characterization of the protein product of bcl-2, the gene involved in human follicular lymphoma

Oncogene. 1987;2(1):3-7.


A protein corresponding in size to the bcl-2 alpha protein obtained after in vitro transcription and in vitro translation in a rabbit reticulocyte lysate was specifically immunoprecipitated from a human B-cell extract using rabbit polyclonal antiserum raised against a beta-galactosidase/bcl-2 fusion protein. Peptide mapping with V8 protease confirmed the identity of this protein as human bcl-2 alpha. Subcellular fractionation of cellular protein followed by immunoprecipitation showed that the bcl-2 alpha protein is associated with the cell membrane.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • B-Lymphocytes / physiology*
  • Cell Compartmentation
  • Cloning, Molecular
  • DNA, Neoplasm / genetics*
  • Genes*
  • Humans
  • Immunologic Techniques
  • Lymphoma / genetics*
  • Membrane Proteins / analysis*
  • Membrane Proteins / genetics
  • Peptide Mapping
  • Proto-Oncogene Proteins / isolation & purification*
  • Proto-Oncogene Proteins c-bcl-2
  • Recombinant Fusion Proteins / analysis
  • Translocation, Genetic


  • DNA, Neoplasm
  • Membrane Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Recombinant Fusion Proteins
  • Bcl2 protein, mouse