Antifeedant and antiphytopathogenic metabolites from co-culture of endophyte Irpex lacteus, phytopathogen Nigrospora oryzae, and entomopathogen Beauveria bassiana

Hai-Yue Yin; Xue-Qiong Yang; Dai-Li Wang; Tong-De Zhao; Cui-Fang Wang; Ya-Bin Yang; Zhong-Tao Ding

doi:10.1016/j.fitote.2020.104781

Antifeedant and antiphytopathogenic metabolites from co-culture of endophyte Irpex lacteus, phytopathogen Nigrospora oryzae, and entomopathogen Beauveria bassiana

Fitoterapia. 2021 Jan;148:104781. doi: 10.1016/j.fitote.2020.104781. Epub 2020 Nov 28.

Authors

Hai-Yue Yin¹, Xue-Qiong Yang¹, Dai-Li Wang¹, Tong-De Zhao¹, Cui-Fang Wang¹, Ya-Bin Yang², Zhong-Tao Ding³

Affiliations

¹ Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, People's Republic of China.
² Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, People's Republic of China. Electronic address: ybyang@ynu.edu.cn.
³ Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, People's Republic of China. Electronic address: ztding@ynu.edu.cn.

PMID: 33259889
DOI: 10.1016/j.fitote.2020.104781

Abstract

Five new tremulane sesquiterpenoids were isolated from co-culture of endophyte Irpex lacteus, phytopathogen Nigrospora oryzae, and entomopathogen Beauveria bassiana. All compounds showed obvious antifeedant activities against silkworm with inhibition percentages of 73-99%, at concentrations of 50 μg/cm². Compound 11 indicated notable antifeedant activity with inhibition percentage of 93% at concentration of 6.25 μg/cm² among them. Compounds 2, 3, 4, 8, 9, 15 and 16 indicated anti-fungal activities against I. lacteus with MIC values ≤8 μg/mL, compounds 11, 12, 16-18 showed significant anti-fungal activity against N. oryzae with MICs ≤ 4 μg/mL, and compounds 2, 5, 12 and 18 indicated significant anti-fungal activity against B. bassiana with MICs ≤ 8 μg/mL. In addition, the I. lacteus should unite B. bassiana to inhibit the production of phytotoxins from N. oryzae in the ternary culture.

Keywords: Anti-fungal activity; Antifeedant activity; Biological function; Co-culture; Metabolite; Triadic relation.

MeSH terms

Animals
Ascomycota / chemistry*
Ascomycota / drug effects
Beauveria / chemistry*
Bombyx / drug effects*
China
Coculture Techniques
Dendrobium / microbiology
Endophytes / chemistry
Fermentation
Fungicides, Industrial / isolation & purification
Fungicides, Industrial / pharmacology*
Microbial Sensitivity Tests
Molecular Structure
Polyporales / chemistry*
Polyporales / drug effects
Seeds / microbiology
Sesquiterpenes / isolation & purification
Sesquiterpenes / pharmacology*

Substances

Fungicides, Industrial
Sesquiterpenes

Supplementary concepts

Irpex lacteus
Nigrospora oryzae