Antifeedant and antiphytopathogenic metabolites from co-culture of endophyte Irpex lacteus, phytopathogen Nigrospora oryzae, and entomopathogen Beauveria bassiana

Fitoterapia. 2021 Jan;148:104781. doi: 10.1016/j.fitote.2020.104781. Epub 2020 Nov 28.

Abstract

Five new tremulane sesquiterpenoids were isolated from co-culture of endophyte Irpex lacteus, phytopathogen Nigrospora oryzae, and entomopathogen Beauveria bassiana. All compounds showed obvious antifeedant activities against silkworm with inhibition percentages of 73-99%, at concentrations of 50 μg/cm2. Compound 11 indicated notable antifeedant activity with inhibition percentage of 93% at concentration of 6.25 μg/cm2 among them. Compounds 2, 3, 4, 8, 9, 15 and 16 indicated anti-fungal activities against I. lacteus with MIC values ≤8 μg/mL, compounds 11, 12, 16-18 showed significant anti-fungal activity against N. oryzae with MICs ≤ 4 μg/mL, and compounds 2, 5, 12 and 18 indicated significant anti-fungal activity against B. bassiana with MICs ≤ 8 μg/mL. In addition, the I. lacteus should unite B. bassiana to inhibit the production of phytotoxins from N. oryzae in the ternary culture.

Keywords: Anti-fungal activity; Antifeedant activity; Biological function; Co-culture; Metabolite; Triadic relation.

MeSH terms

  • Animals
  • Ascomycota / chemistry*
  • Ascomycota / drug effects
  • Beauveria / chemistry*
  • Bombyx / drug effects*
  • China
  • Coculture Techniques
  • Dendrobium / microbiology
  • Endophytes / chemistry
  • Fermentation
  • Fungicides, Industrial / isolation & purification
  • Fungicides, Industrial / pharmacology*
  • Microbial Sensitivity Tests
  • Molecular Structure
  • Polyporales / chemistry*
  • Polyporales / drug effects
  • Seeds / microbiology
  • Sesquiterpenes / isolation & purification
  • Sesquiterpenes / pharmacology*

Substances

  • Fungicides, Industrial
  • Sesquiterpenes

Supplementary concepts

  • Irpex lacteus
  • Nigrospora oryzae