A Case-Study of the Molecular Diagnosis of Echinococcus multilocularis in Wild Boar with Comments on its Public Health Significance in Turkey

J Parasitol. 2020 Nov 12;106(6):730-734. doi: 10.1645/19-196.

Abstract

Echinococcus multilocularis is a parasite species of zoonotic importance which can be fatal to humans and causes Alveolar Echinococcosis (AE). This report describes the development of a cyst from the liver of a wild boar and molecular confirmation of its identification. The cyst material was obtained from the liver of a wild boar killed by hunters. Genomic DNA was extracted from the germinal layer of the cyst material, and 875 bp mitochondrial cytochrome c oxidase subunit I (COI) gene fragment was amplified by PCR and sequenced. A BLAST search matched 100% with published Echinococcus multilocularis sequences. This study confirms the occurrence of E. multilocularis in a wild boar for the first time in Turkey.

Keywords: Echinococcus multilocularis; PCR; Sequencing; Turkey; Wild Boar.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Helminth / genetics
  • DNA, Helminth / isolation & purification
  • Echinococcosis, Hepatic / diagnosis
  • Echinococcosis, Hepatic / parasitology
  • Echinococcosis, Hepatic / pathology
  • Echinococcosis, Hepatic / veterinary*
  • Echinococcus multilocularis / classification
  • Echinococcus multilocularis / genetics
  • Echinococcus multilocularis / isolation & purification*
  • Liver / parasitology
  • Phylogeny
  • Sus scrofa / parasitology*
  • Swine
  • Swine Diseases / diagnosis*
  • Swine Diseases / parasitology*
  • Swine Diseases / pathology
  • Turkey

Substances

  • DNA, Helminth