MIR155HG Knockdown Inhibited the Progression of Cervical Cancer by Binding SRSF1

Ling Shen; Yuancheng Li; Guiying Hu; Yihong Huang; Xinli Song; Shun Yu; Xiaoyuan Xu

doi:10.2147/OTT.S267594

MIR155HG Knockdown Inhibited the Progression of Cervical Cancer by Binding SRSF1

Onco Targets Ther. 2020 Nov 23:13:12043-12054. doi: 10.2147/OTT.S267594. eCollection 2020.

Authors

Ling Shen¹, Yuancheng Li², Guiying Hu³, Yihong Huang², Xinli Song⁴, Shun Yu⁴, Xiaoyuan Xu¹

Affiliations

¹ Department of Obstetrics and Gynecology, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, People's Republic of China.
² Department of Gynecological Oncology, The Affiliated Cancer Hospital of Shantou University Medical College, Shantou, Guangdong 515041, People's Republic of China.
³ Department of Gynecology, Maternal and Children Hospital of Guangdong Province, Guangzhou, Guangdong 511400, People's Republic of China.
⁴ Department of Gynecological Oncology, The Affiliated Cancer Hospital of Shantou University Medical College, Shantou, Guangdong, 515041, People's Republic of China.

Abstract

Background: As the fourth most common cancer among women worldwide, cervical cancer lead to 311,000 deaths in 2018. Although the treatments have been developed, the survival rate of cervical cancer remains unsatisfactory. In this study, we aimed to identify differentially expressed lncRNAs (DEIncRNAs) between cervical cancer and adjacent normal tissues using bioinformatics analysis, and further to investigate the biological function of the DEIncRNAs in vitro and in vivo.

Methods: The expression profiles from two microarray datasets (GSE6791 and GSE63514) were downloaded from GEO for analysis of DEIncRNAs between cervical cancer and adjacent normal cervical tissues. Among all DEIncRNAs, MIR155HG upregulation was identified and selected for further investigation. The effect of MIR155HG knockdown on proliferation, apoptosis and invasion in SiHa and Hela cells were evaluated. In addition, Western blot, RNA immunoprecipitation (RIP) and cell cycle assays were performed to determine the binding target of MIR155HG. Furthermore, the effect of MIR155HG knockdown on tumor growth in vivo was investigated.

Results: The level of MIR155HG was found to be significantly upregulated in cervical cancer tissue compared with adjacent cervical tissue. Knockdown of MIR155HG notably inhibited the proliferation of SiHa and Hela cells by inducing apoptosis. In addition, MIR155HG knockdown decreased cell invasion. Moreover, tumor growth in xenograft was significantly inhibited by MIR155HG knockdown in vivo. Additionally, SRSF1 was identified as the binding protein of MIR155HG.

Conclusion: Our findings demonstrated that MIR155HG knockdown inhibited the progression of cervical cancer by binding SRSF1, inspiring the usage of MIR155HG as a potential novel therapy target for the treatment of cervical cancer.

Keywords: MIR155HG; SRSF1; cervical cancer.

Grants and funding

This study was supported by Natural Science Foundation doctoral program of Guangdong Provincial Science and Technology Department. The role of HIV gp120 in apoptosis of sperm. Grant No.: 2016a030310075.