Heterogeneity of T Cells in Atherosclerosis Defined by Single-Cell RNA-Sequencing and Cytometry by Time of Flight

Arterioscler Thromb Vasc Biol. 2021 Feb;41(2):549-563. doi: 10.1161/ATVBAHA.120.312137. Epub 2020 Dec 3.

Abstract

The infiltration and accumulation of pro- and anti-inflammatory leukocytes within the intimal layer of the arterial wall is a hallmark of developing and progressing atherosclerosis. While traditionally perceived as macrophage- and foam cell-dominated disease, it is now established that atherosclerosis is a partial autoimmune disease that involves the recognition of peptides from ApoB (apolipoprotein B), the core protein of LDL (low-density lipoprotein) cholesterol particles, by CD4+ T-helper cells and autoantibodies against LDL and ApoB. Autoimmunity in the atherosclerotic plaque has long been understood as a pathogenic T-helper type-1 driven response with proinflammatory cytokine secretion. Recent developments in high-parametric cell immunophenotyping by mass cytometry, single-cell RNA-sequencing, and in tools exploring antigen-specificity have established the existence of several unforeseen layers of T-cell diversity with mixed TH1 and T regulatory cells transcriptional programs and unpredicted fates. These findings suggest that pathogenic ApoB-reactive T cells evolve from atheroprotective and immunosuppressive CD4+ T regulatory cells that lose their protective properties over time. Here, we discuss T-cell heterogeneity in atherosclerosis with a focus on plasticity, antigen-specificity, exhaustion, maturation, tissue residency, and its potential use in clinical prediction.

Keywords: atherosclerosis; autoantibodies; autoimmunity; immunophenotyping; peptide.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Atherosclerosis* / genetics
  • Atherosclerosis* / immunology
  • Atherosclerosis* / metabolism
  • Atherosclerosis* / pathology
  • Autoimmunity
  • Biomarkers / metabolism
  • Cell Lineage
  • Cell Separation*
  • Flow Cytometry*
  • Gene Expression Profiling*
  • Humans
  • Immunophenotyping
  • Inflammation* / genetics
  • Inflammation* / immunology
  • Inflammation* / metabolism
  • Inflammation* / pathology
  • Phenotype
  • Plaque, Atherosclerotic
  • RNA-Seq*
  • Single-Cell Analysis*
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism*
  • Transcriptome*

Substances

  • Biomarkers