Mycobacterium vaccae Lysate Induces Anti-Allergic Immune Response In Vitro

Bull Exp Biol Med. 2020 Dec;170(2):226-229. doi: 10.1007/s10517-020-05039-6. Epub 2020 Dec 3.


Mycobacterium vaccae is a soil saprophyte which exerts anti-allergic properties. There are data that mechanism of action of M. vaccae when used in the treatment of human and animal allergic diseases is associated with Th1-phenotype switch. Here we studied the properties of sonicated M. vaccae lysate in co-cultures of dendritic cells and CD4+T cells. M. vaccae lysate stimulated IL-10 synthesis in co-cultures and CD86 expression in dendritic cells, being more potent than heat-killed M. vaccae. The reported clinical data and the mechanism of action of M. vaccae lysate suggest that its use is a feasible option for the primary prevention of allergic diseases, in particular atopic dermatitis.

Keywords: atopic dermatitis; bacterial lysates; cytokines; dendritic cells; mycobacterium.

MeSH terms

  • Animals
  • Anti-Allergic Agents
  • B7-2 Antigen / metabolism
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • Cytokines / biosynthesis
  • Dendritic Cells / metabolism
  • Dermatitis, Atopic
  • Humans
  • Hypersensitivity / immunology*
  • Immunity
  • In Vitro Techniques
  • Interleukin-10 / metabolism
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mycobacteriaceae*
  • Phenotype
  • Th1 Cells / microbiology*
  • Young Adult


  • Anti-Allergic Agents
  • B7-2 Antigen
  • CD86 protein, human
  • Cytokines
  • IL10 protein, human
  • Interleukin-10

Supplementary concepts

  • Mycolicibacterium vaccae