Yeast arginine permease: nucleotide sequence of the CAN1 gene

Curr Genet. 1986;10(8):587-92. doi: 10.1007/BF00418125.

Abstract

The yeast CAN1 gene, thought to encode arginine permease, has found use in genetics as a selectable locus. We have sequenced the cloned CAN1 gene, which contains an open reading frame of 1770 nucleotides, encoding a polypeptide of calculated molecular weight 65,766. Disruption of this open reading frame largely abolishes CAN1 gene expression, while subcloned fragments of the open reading frame hybridize strand-specifically to a 2.3 kb yeast RNA message. The encoded protein has no leader signal sequence, and is highly hydrophobic, with a possible twelve membrane-spanning domains, several of which have the high hydrophobic moments seen in channel-forming or permease proteins. This protein structure is consistent with the CAN1 product being the plasma membrane arginine permease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Transport Systems*
  • Amino Acid Transport Systems, Basic
  • Base Sequence
  • Cloning, Molecular
  • Genes
  • Genes, Fungal*
  • Membrane Proteins / genetics
  • Membrane Transport Proteins / genetics*
  • Molecular Sequence Data
  • Protein Conformation
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins
  • Solubility
  • Transcription, Genetic

Substances

  • Amino Acid Transport Systems
  • Amino Acid Transport Systems, Basic
  • CAN1 protein, S cerevisiae
  • Membrane Proteins
  • Membrane Transport Proteins
  • Saccharomyces cerevisiae Proteins
  • arginine permease

Associated data

  • GENBANK/X03784