Experimental challenge of a North American bat species, big brown bat (Eptesicus fuscus), with SARS-CoV-2

doi:10.1111/tbed.13949

. 2021 Nov;68(6):3443-3452.

doi: 10.1111/tbed.13949. Epub 2021 Jan 11.

Experimental challenge of a North American bat species, big brown bat (Eptesicus fuscus), with SARS-CoV-2

Affiliations

¹ U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA.
² Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
³ Louisiana National Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.

PMID: 33295095
DOI: 10.1111/tbed.13949

Experimental challenge of a North American bat species, big brown bat (Eptesicus fuscus), with SARS-CoV-2

Jeffrey S Hall et al. Transbound Emerg Dis. 2021 Nov.

. 2021 Nov;68(6):3443-3452.

doi: 10.1111/tbed.13949. Epub 2021 Jan 11.

Authors

Affiliations

¹ U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA.
² Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
³ Louisiana National Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.

PMID: 33295095
DOI: 10.1111/tbed.13949

Abstract

The recently emerged novel coronavirus, SARS-CoV-2, is phylogenetically related to bat coronaviruses (CoVs), specifically SARS-related CoVs from the Eurasian bat family Rhinolophidae. As this human pandemic virus has spread across the world, the potential impacts of SARS-CoV-2 on native North American bat populations are unknown, as is the ability of North American bats to serve as reservoirs or intermediate hosts able to transmit the virus to humans or to other animal species. To help determine the impacts of the pandemic virus on North American bat populations, we experimentally challenged big brown bats (Eptesicus fuscus) with SARS-CoV-2 under BSL-3 conditions. We inoculated the bats both oropharyngeally and nasally, and over the ensuing three weeks, we measured infectivity, pathology, virus concentrations in tissues, oral and rectal virus excretion, virus transmission, and clinical signs of disease. We found no evidence of SARS-CoV-2 infection in any examined bat, including no viral excretion, no transmission, no detectable virus in tissues, and no signs of disease or pathology. Based on our findings, it appears that big brown bats are resistant to infection with the SARS-CoV-2. The potential susceptibility of other North American bat species to SARS-CoV-2 remains to be investigated.

Keywords: SARS-CoV-2; big brown bats; challenge; coronavirus; susceptibility.

Published 2020. This article is a U.S. Government work and is in the public domain in the USA.

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References

REFERENCES

1. Bartlett, S. L., Diel, D. G., Wang, L., Zec, S., Laverack, M., Martins, M., Caserta, L. C., Killian, M. L., Terio, K., Olmstead, C., Delaney, M. A., Stokol, T., Ivančić, M., Jenkins-Moore, M., Ingerman, K., Teegan, T., McCann, C., Thomas, P., McAloose, D., &Calle, P. P. (2020). SARS-CoV-2 infection in Malayan tigers (Panthera tigris Jacksoni), Amur tigers (Panthera tigris Altaica), and African lions (Panthera leo Krugeri) at the Bronx Zoo, New York, USA. bioRxiv. https://doi.org/10.1101/2020.08.14.250928
1. Becker, D. J., Albery, G. F., Sjodin, A. R., Poisot, T., Dallas, T. A., Eskew, E. A., Farrell, M. J., Guth, S., Han, B. A., Simmons, N. B., & Carlson, C. J. (2020). Trends Ecol Evol., 35(12), 1062-1065. https://doi.org/10.1101/2020.05.22.111344
1. Blehert, D. S., Hicks, A. C., Behr, M., Meteyer, C. U., Berlowski-Zier, B. M., Buckles, E. L., Coleman, J. T. H., Darling, S. R., Gargas, A., Niver, R., Okoniewski, J. C., Rudd, R. J., & Stone, W. B. (2009). Bat white-nose syndrome: An emerging fungal pathogen? Science, 323, 227. https://doi.org/10.1126/science.1163874
1. Boni, M. F., Lemey, P., Jiang, X., Lam, T.-Y., Perry, B. W., Castoe, T. A., Rambaut, A., & Robertson, D. L. (2020). Evolutionary origins of the SARS-CoV-2 sarbecovirus lineage responsible for the COVID-19 pandemic. Nature Microbiology, 5(11), 1408-1417. https://doi.org/10.1038/s41564-020-0771-4
1. Carossino, M., Dini, P., Kalbfleisch, T. S., Loynachan, A. T., Canisso, I. F., Cook, R. F., Timoney, P. J., & Balasuriya, U. B. R. (2019). Equine arteritis virus long-term persistence is orchestrated by CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis. PLoS Path, 15(7), e1007950. https://doi.org/10.1371/journal.ppat.1007950

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[1] Bartlett, S. L., Diel, D. G., Wang, L., Zec, S., Laverack, M., Martins, M., Caserta, L. C., Killian, M. L., Terio, K., Olmstead, C., Delaney, M. A., Stokol, T., Ivančić, M., Jenkins-Moore, M., Ingerman, K., Teegan, T., McCann, C., Thomas, P., McAloose, D., &Calle, P. P. (2020). SARS-CoV-2 infection in Malayan tigers (Panthera tigris Jacksoni), Amur tigers (Panthera tigris Altaica), and African lions (Panthera leo Krugeri) at the Bronx Zoo, New York, USA. bioRxiv. https://doi.org/10.1101/2020.08.14.250928

[2] Bartlett, S. L., Diel, D. G., Wang, L., Zec, S., Laverack, M., Martins, M., Caserta, L. C., Killian, M. L., Terio, K., Olmstead, C., Delaney, M. A., Stokol, T., Ivančić, M., Jenkins-Moore, M., Ingerman, K., Teegan, T., McCann, C., Thomas, P., McAloose, D., &Calle, P. P. (2020). SARS-CoV-2 infection in Malayan tigers (Panthera tigris Jacksoni), Amur tigers (Panthera tigris Altaica), and African lions (Panthera leo Krugeri) at the Bronx Zoo, New York, USA. bioRxiv. https://doi.org/10.1101/2020.08.14.250928

[3] Becker, D. J., Albery, G. F., Sjodin, A. R., Poisot, T., Dallas, T. A., Eskew, E. A., Farrell, M. J., Guth, S., Han, B. A., Simmons, N. B., & Carlson, C. J. (2020). Trends Ecol Evol., 35(12), 1062-1065. https://doi.org/10.1101/2020.05.22.111344

[4] Becker, D. J., Albery, G. F., Sjodin, A. R., Poisot, T., Dallas, T. A., Eskew, E. A., Farrell, M. J., Guth, S., Han, B. A., Simmons, N. B., & Carlson, C. J. (2020). Trends Ecol Evol., 35(12), 1062-1065. https://doi.org/10.1101/2020.05.22.111344

[5] Blehert, D. S., Hicks, A. C., Behr, M., Meteyer, C. U., Berlowski-Zier, B. M., Buckles, E. L., Coleman, J. T. H., Darling, S. R., Gargas, A., Niver, R., Okoniewski, J. C., Rudd, R. J., & Stone, W. B. (2009). Bat white-nose syndrome: An emerging fungal pathogen? Science, 323, 227. https://doi.org/10.1126/science.1163874

[6] Blehert, D. S., Hicks, A. C., Behr, M., Meteyer, C. U., Berlowski-Zier, B. M., Buckles, E. L., Coleman, J. T. H., Darling, S. R., Gargas, A., Niver, R., Okoniewski, J. C., Rudd, R. J., & Stone, W. B. (2009). Bat white-nose syndrome: An emerging fungal pathogen? Science, 323, 227. https://doi.org/10.1126/science.1163874

[7] Boni, M. F., Lemey, P., Jiang, X., Lam, T.-Y., Perry, B. W., Castoe, T. A., Rambaut, A., & Robertson, D. L. (2020). Evolutionary origins of the SARS-CoV-2 sarbecovirus lineage responsible for the COVID-19 pandemic. Nature Microbiology, 5(11), 1408-1417. https://doi.org/10.1038/s41564-020-0771-4

[8] Boni, M. F., Lemey, P., Jiang, X., Lam, T.-Y., Perry, B. W., Castoe, T. A., Rambaut, A., & Robertson, D. L. (2020). Evolutionary origins of the SARS-CoV-2 sarbecovirus lineage responsible for the COVID-19 pandemic. Nature Microbiology, 5(11), 1408-1417. https://doi.org/10.1038/s41564-020-0771-4

[9] Carossino, M., Dini, P., Kalbfleisch, T. S., Loynachan, A. T., Canisso, I. F., Cook, R. F., Timoney, P. J., & Balasuriya, U. B. R. (2019). Equine arteritis virus long-term persistence is orchestrated by CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis. PLoS Path, 15(7), e1007950. https://doi.org/10.1371/journal.ppat.1007950

[10] Carossino, M., Dini, P., Kalbfleisch, T. S., Loynachan, A. T., Canisso, I. F., Cook, R. F., Timoney, P. J., & Balasuriya, U. B. R. (2019). Equine arteritis virus long-term persistence is orchestrated by CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis. PLoS Path, 15(7), e1007950. https://doi.org/10.1371/journal.ppat.1007950

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Experimental challenge of a North American bat species, big brown bat (Eptesicus fuscus), with SARS-CoV-2

Affiliations

Experimental challenge of a North American bat species, big brown bat (Eptesicus fuscus), with SARS-CoV-2

Authors

Affiliations

Abstract

Similar articles

Cited by

References

REFERENCES

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical

Research Materials

Miscellaneous