Isolation and Purification of DNA from Complicated Biological Samples

Methods Mol Biol. 2021:2222:57-67. doi: 10.1007/978-1-0716-0997-2_3.


The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi, and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.

Keywords: Animal tissues; CTAB; DNA extraction; Food; Herbarium specimens; High-quality DNA; Plant tissues; Soil; Woody plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chemical Fractionation / methods*
  • DNA / analysis
  • DNA / isolation & purification*
  • Food
  • Molecular Biology / methods
  • Plants / genetics
  • Soil / chemistry
  • Spectrophotometry


  • Soil
  • DNA