Induction of scaffold angiogenesis by recipient vasculature precision micropuncture

Microvasc Res. 2021 Mar;134:104121. doi: 10.1016/j.mvr.2020.104121. Epub 2020 Dec 11.

Abstract

The success of engineered tissues continues to be limited by time to vascularization and perfusion. Here, we studied the effects of precision injury to a recipient macrovasculature in promoting neovessel formation in an adjacently placed scaffold. Segmental 60 μm diameter micropunctures (MP) were created in the recipient rat femoral artery and vein followed by coverage with a simple collagen scaffold. Scaffolds were harvested at 24, 48, 72, and 96 h post-implantation for detailed analysis. Those placed on top of an MP segment showed an earlier and more robust cellular infiltration, including both endothelial cells (CD31) and macrophages (F4/80), compared to internal non-micropunctured control limbs (p < 0.05). At the 96-hour timepoint, MP scaffolds demonstrated an increase in physiologic perfusion (p < 0.003) and a 2.5-fold increase in capillary network formation (p < 0.001). These were attributed to an overall upsurge in small vessel quantity. Furthermore, MP positioned scaffolds demonstrated significant increases in many modulators of angiogenesis, including VEGFR2 and Tie-2 despite a decrease in HIF-1α at all timepoints. This study highlights a novel microsurgical approach that can be used to rapidly vascularize or inosculate contiguously placed scaffolds and grafts. Thereby, offering an easily translatable route towards the creation of thicker and more clinically relevant engineered tissues.

Keywords: Angiogenesis; Collagen; Graft; Micropuncture; Scaffold; Surgery; Vascularization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Animals
  • Collagen / metabolism
  • Femoral Artery* / metabolism
  • Femoral Vein* / metabolism
  • Hindlimb / blood supply*
  • Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
  • Neovascularization, Physiologic*
  • Punctures
  • Rats, Sprague-Dawley
  • Receptor, TIE-2 / metabolism
  • Signal Transduction
  • Time Factors
  • Tissue Engineering*
  • Tissue Scaffolds*
  • Vascular Endothelial Growth Factor Receptor-2 / metabolism

Substances

  • Hif1a protein, rat
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Collagen
  • Kdr protein, rat
  • Receptor, TIE-2
  • Vascular Endothelial Growth Factor Receptor-2