Immobilized phosphate-binding protein can effectively discriminate against arsenate during phosphate adsorption and recovery

Abstract

There is a strong impetus to establish a circular phosphorus economy by securing internally renewable phosphate (P_i ) resources for use as agricultural fertilizers. Reversible P_i adsorption technologies such as ion exchange can remove and recover P_i from water/wastewater for reuse. However, existing reversible adsorbents cannot effectively discriminate against arsenate (As(V)) due to the similarity between As(V) and P_i chemical structure. If As(V) is co-recovered with P_i , the value of the recovered products for agricultural reuse is low. The objective of this study was to construct an immobilized phosphate-binding protein (PBP)-based P_i removal and recovery system and analyze its selectivity for P_i adsorption in the presence of As(V). A range of conditions was tested, including independent, sequential, and simultaneous exposure of the two oxyanions to immobilized PBP (PBP resin). The purity of the recovered P_i product was assessed after inducing controlled desorption of the adsorbed oxyanions at high pH (pH 12.5). P_i constituted more than 97% of the adsorbed oxyanions in the recovered product, even when As(V) was initially present at twofold higher concentrations than P_i . Therefore, PBP resin has potential to selectively remove P_i , as well as release high-purity P_i free of As(V) contamination suitable for subsequent agricultural reuse. PRACTITIONER POINTS: Existing reversible phosphate (P_i ) adsorbents cannot effectively discriminate against arsenate (As(V)) due to the similarity in their chemical structure. Co-recovery of As(V) with P_i can reduce the recovered product's reuse as a fertilizer. An immobilized phosphate-binding protein (PBP)-based system can be highly selective for P_i even in the presence of As(V). P_i constituted more than 97% of the recovered product, even when As(V) was present at 2-fold higher concentrations than P_i . Immobilized PBP offers advantages over existing P_i adsorbents by providing high-purity P_i products free of As(V) contamination for reuse.

Keywords: adsorption; resource recovery; surface water; wastewater treatment.