3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening

ACS Biomater Sci Eng. 2020 Dec 14;6(12):6701-6709. doi: 10.1021/acsbiomaterials.0c01468. Epub 2020 Nov 13.

Abstract

The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being induced by a kidney extracellular matrix. Levels of CYP2E1 and KIM-1 in 3-D organoids were significantly increased in response to acetone and cisplatin. This 3-D culture system provides an alternative tool for nephrotoxicity screening and research.

Keywords: 3d culture; drug testing; kidney extracellular matrix; renal progenitor cells.

MeSH terms

  • Cell Differentiation
  • Cisplatin
  • Humans
  • Kidney*
  • Organoids*
  • Stem Cells

Substances

  • Cisplatin