Anti-drug antibody detection with label-free electrolyte-gated organic field-effect transistors

Chem Commun (Camb). 2021 Jan 14;57(3):367-370. doi: 10.1039/d0cc03399e.


The efficacy of immunotherapy can be undermined by the development of an immune response against a drug/antibody mediated by anti-drug antibodies (ADAs) in treated patients. We present the first label-free EGOFET immunosensor that integrates a biological drug, Nivolumab (Opdivo©), as a specific recognition moiety to quantitatively and selectively detect ADAs against the drug. The limit of detection is 100 fM. This demonstration is a prelude to the detection of ADAs in a clinical setting in the treatment of different pathologies, and it also enables rapid screening of biological drugs for immunogenicity.

MeSH terms

  • Antibodies, Monoclonal / analysis*
  • Antibodies, Monoclonal / immunology
  • Electrolytes / chemistry
  • Humans
  • Nivolumab / immunology*
  • Transistors, Electronic*


  • Antibodies, Monoclonal
  • Electrolytes
  • Nivolumab