A novel function of African Swine Fever Virus pE66L in inhibition of host translation by the PKR/eIF2α pathway

Zhou Shen; Chen Chen; Yilin Yang; Zhenhua Xie; Qingying Ao; Lu Lv; Shoufeng Zhang; Huanchun Chen; Rongliang Hu; Hongjun Chen; Guiqing Peng

doi:10.1128/JVI.01872-20

A novel function of African Swine Fever Virus pE66L in inhibition of host translation by the PKR/eIF2α pathway

J Virol. 2021 Mar 1;95(5):e01872-20. doi: 10.1128/JVI.01872-20. Epub 2020 Dec 16.

Authors

Zhou Shen¹, Chen Chen¹, Yilin Yang¹, Zhenhua Xie², Qingying Ao², Lu Lv², Shoufeng Zhang³, Huanchun Chen^{1

4}, Rongliang Hu³, Hongjun Chen², Guiqing Peng^{5

4}

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
² Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
³ Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China.
⁴ Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
⁵ State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China penggq@mail.hzau.edu.cn.

Abstract

African swine fever virus (ASFV) is one of the most contagious and lethal viruses infecting pigs. This virus is endemic in many countries and has very recently spread to China, but no licensed vaccines or treatments are currently available. Despite extensive research, the basic question of how ASFV-encoded proteins inhibit host translation remains. Here, we examined how ASFV interfered with host translation and optimized viral gene expression. We found that 14 ASFV proteins inhibited Renilla luciferase (Rluc) activity greater than 5-fold, and the protein with the strongest inhibitory effect was pE66L, which was not previously reported. Combined with bioinformatical analysis and biochemical experiment, we determined that the transmembrane (TM) domain (amino acids 13-34) of pE66L was required for the inhibition of host gene expression. Notably, we constructed a recombinant plasmid with the TM domain linked to enhanced green fluorescent protein (EGFP) and further demonstrated that this domain broadly inhibited protein synthesis. Confocal and biochemical analyses indicated that the TM domain might help proteins locate to the endoplasmic reticulum (ER) to suppress translation though the PKR/eIF2α pathway. Deletion of the E66L gene had little effect on virus replication in macrophages, but significantly recovered host gene expression. Taken together, our findings complement studies on the host translation of ASFV proteins and suggest that ASFV pE66L induces host translation shutoff, which is dependent on activation of the PKR/eIF2α pathway.Importance African swine fever virus (ASFV) is a member of the nucleocytoplasmic large DNA virus superfamily that predominantly replicates in the cytoplasm of infected cells. The ASFV double-stranded DNA genome varies in length from approximately 170 to 193 kbp depending on the isolate and contains between 150 and 167 open reading frames (ORFs), of which half the encoded proteins have not been explored. Our study showed that 14 proteins had an obvious inhibitory effect on Renilla luciferase (Rluc) gene synthesis, with pE66L showing the most significant effect. Furthermore, the transmembrane (TM) domain of pE66L broadly inhibited host protein synthesis in a PKR/eIF2a pathway-dependent manner. Loss of pE66L during ASFV infection had little effect on virus replication, but significantly recovered host protein synthetic. Based on the above results, our findings expand our view of ASFV in determining the fate of host-pathogen interactions.