Using convalescent plasma as immunotherapy is an old method for treatment of infectious diseases. Several countries have recently allowed the use of such therapy for the treatment of COVID-19 patients especially those who are critically ill. A similar program is currently being tested in Egypt. Here, we tested 227 plasma samples from convalescent donors in Egypt for neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using a microneutralization (MN) assay. A third of the tested samples did not have antibody titers and 58% had titers between 1:10 and 1:80. Only 12% had titers >1:160. We also compared MN assays using different virus concentrations, plaque reduction neutralization (PRNT) assays, and a chemiluminescence assay that measures immunoglobulin G (IgG) binding to N and S proteins of SARS-CoV-2. Our results indicated that a MN assay using 100 TCID50/ml provides comparable results to PRNT and allows for high throughput testing.
Keywords: Egypt; microneutralization assay; neutralizing antibodies; plasma donors; severe acute respiratory syndrome coronavirus 2.
Copyright © 2020 Gomaa, Kandeil, Mostafa, Roshdy, Kayed, Shehata, Kutkat, Moatasim, El Taweel, Mahmoud, Kamel, Abo Shama, El Sayes, El-Shesheny, Bakheet, Elgohary, Elbadry, Nassif, Ahmed, Abdel Messih, Kayali and Ali.