Actin and calmodulin coexist in the equatorial segment of ejaculated boar sperm

Gamete Res. 1987 Jun;17(2):97-105. doi: 10.1002/mrd.1120170202.

Abstract

The direct overlay technique with iodinated calmodulin on boar sperm fractions evidenced a strong bond in the 45,000 molecular weight, which is the region recognized by anti-actin antibodies. This result and the close codistribution of the two staining patterns for calmodulin and actin with immunofluorescence and immunoelectronmicroscopy suggest a possible interaction between calmodulin and actin in boar sperm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / analysis*
  • Animals
  • Calmodulin / analysis*
  • Calmodulin-Binding Proteins / analysis
  • Ejaculation
  • Fluorescent Antibody Technique
  • Male
  • Microscopy, Electron
  • Molecular Weight
  • Spermatozoa / analysis*
  • Spermatozoa / cytology
  • Spermatozoa / ultrastructure
  • Swine

Substances

  • Actins
  • Calmodulin
  • Calmodulin-Binding Proteins