Biological nitrogen and phosphorus removal by a phosphorus-accumulating bacteria Acinetobacter sp. strain C-13 with the ability of heterotrophic nitrification-aerobic denitrification

Huanjun Chen; Weizheng Zhou; Shunni Zhu; Fen Liu; Lei Qin; Chao Xu; Zhongming Wang

doi:10.1016/j.biortech.2020.124507

Biological nitrogen and phosphorus removal by a phosphorus-accumulating bacteria Acinetobacter sp. strain C-13 with the ability of heterotrophic nitrification-aerobic denitrification

Bioresour Technol. 2021 Feb:322:124507. doi: 10.1016/j.biortech.2020.124507. Epub 2020 Dec 8.

Authors

Huanjun Chen¹, Weizheng Zhou², Shunni Zhu², Fen Liu¹, Lei Qin², Chao Xu³, Zhongming Wang⁴

Affiliations

¹ Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China; University of China Academy of Sciences, Beijing 100049, China.
² Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.
³ Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
⁴ Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China. Electronic address: wangzm@ms.giec.ac.cn.

PMID: 33338941
DOI: 10.1016/j.biortech.2020.124507

Abstract

Strain C-13, identified as an Acinetobacter sp. by homology searches, exhibited efficient simultaneous heterotrophic nitrification-aerobic denitrification phosphorus removal (SNDPR) abilities by nitrogen balance analysis and further confirmation of successful amplification of functional genes ppk, napA, and nirS. In addition, strain C-13 could utilize NH₄⁺-N, NO₃^--N, and NO₂^--N as nitrogen sources, among which NH₄⁺-N was indicated to be an excellent nitrogen source for assimilation and heterotrophic nitrification. Besides, the optimum conditions for nutrient removal were determined as follows: sodium acetate as the sole carbon source, C/N/P ratio of 100/10/2, pH = 7.5, and temperature of 30 °C. Meanwhile, the strain also showed the traditional features, such as release and the excess uptake of phosphate under anaerobic/aerobic conditions, with the highest phosphorus content of 5.01% after cultivation. Strain C-13 presents promising prospects for application in biologicalnutrient removal in wastewater treatment.

Keywords: Acinetobacter; Anaerobic/aerobic; Nitrogen balance analysis; Phosphorus content; SNDPR.

MeSH terms

Acinetobacter*
Aerobiosis
Bacteria
Denitrification
Heterotrophic Processes
Nitrification*
Nitrites
Nitrogen
Phosphorus

Substances

Nitrites
Phosphorus
Nitrogen