Olive Pâté by Multi-Phase Decanter as Potential Source of Bioactive Compounds of Both Nutraceutical and Anticancer Effects

Molecules. 2020 Dec 16;25(24):5967. doi: 10.3390/molecules25245967.


In the oil sector, a novelty in the centrifugal extraction system is represented by the multi-phase decanters (DMF) that work without adding process water and with the advantage of recovering a dried pomace and a by-product, called "pâté", consisting of the pulp and its vegetation water, without traces of stone. The pâté has a high content of phenolic compounds, mainly represented by secoiridoids and verbascoside. The present work investigated the efficacy of two different ways of debittering (by sequential filtrations and spontaneous fermentation) of DMF pâté from three olive cultivars (Olea europaea L. "Leccino", "Carboncella" and "Tortiglione") to make the pâté edible, and, contemporary, investigated also the effect of its phenolic bioactive extracts on pathogenic bacteria and colon cancer cell model. Daily filtrations of pâté of the three cultivars have been shown to be more efficient in phenolic degradation. The activity of the indigenous microflora on the other hand takes a longer time to degrade the phenolic component and therefore to de-bitter it. None of pâté showed antibacterial activity. Colorimetric assay MTS for cell viability and metabolic activity tested on colon cancer cells CaCo2 and HCT116 suggest a potential beneficial effect of the dried extracts probably related to the modulation of gene expression under these treatments.

Keywords: colon cancer cells; multi-phase decanter; olive oil by-products; phenolic compounds; secoiridoids.

MeSH terms

  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Dietary Supplements / analysis*
  • Drug Resistance, Multiple, Bacterial / drug effects
  • Gene Expression Regulation, Neoplastic / drug effects
  • Gram-Negative Bacteria / drug effects
  • Gram-Positive Bacteria / drug effects
  • Humans
  • Iridoids / chemistry
  • Iridoids / isolation & purification
  • Iridoids / pharmacology
  • Liquid-Liquid Extraction
  • Microbial Sensitivity Tests
  • Olea / metabolism*
  • Olive Oil / chemistry*
  • Phenols / chemistry
  • Phenols / isolation & purification
  • Phenols / pharmacology


  • Antineoplastic Agents
  • Iridoids
  • Olive Oil
  • Phenols