Transcriptomic profiles in pulmonary arterial hypertension associate with disease severity and identify novel candidate genes

Casey E Romanoski; Xinshuai Qi; Shreya Sangam; Rebecca R Vanderpool; Robert S Stearman; Austin Conklin; Manuel Gonzalez-Garay; Franz Rischard; Ramon J Ayon; Jian Wang; Tatum Simonson; Aleksandra Babicheva; Yinan Shi; Haiyang Tang; Ayako Makino; Yogendra Kanthi; Mark W Geraci; Joe G N Garcia; Jason X-J Yuan; Ankit A Desai

doi:10.1177/2045894020968531

Transcriptomic profiles in pulmonary arterial hypertension associate with disease severity and identify novel candidate genes

Pulm Circ. 2020 Dec 7;10(4):2045894020968531. doi: 10.1177/2045894020968531. eCollection 2020 Oct-Dec.

Authors

Casey E Romanoski¹, Xinshuai Qi², Shreya Sangam^{2

3}, Rebecca R Vanderpool², Robert S Stearman³, Austin Conklin¹, Manuel Gonzalez-Garay², Franz Rischard², Ramon J Ayon², Jian Wang^{2

4

5}, Tatum Simonson⁵, Aleksandra Babicheva⁵, Yinan Shi³, Haiyang Tang^{2

4}, Ayako Makino^{2

5

6}, Yogendra Kanthi^{7

8}, Mark W Geraci³, Joe G N Garcia^{2

6}, Jason X-J Yuan^{2

5

6}, Ankit A Desai^{2

3}

Affiliations

¹ Department of Cellular and Molecular Medicine, College of Medicine, The University of Arizona, Tucson, AZ, USA.
² Department of Medicine, College of Medicine, The University of Arizona, Tucson, AZ, USA.
³ Department of Medicine, Indiana University, Indianapolis, IN, USA.
⁴ State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, Guangzhou Medical University, Guangzhou, China.
⁵ Department of Medicine, University of California, San Diego, La Jolla, CA, USA.
⁶ Department of Physiology, College of Medicine, The University of Arizona, Tucson, AZ, USA.
⁷ Division of Intramural Research National Heart, Lung and Blood Institute Bethesda, Maryland, USA.
⁸ Division of Cardiovascular Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.

Abstract

Using RNAseq, we identified a 61 gene-based circulating transcriptomic profile most correlated with four indices of pulmonary arterial hypertension severity. In an independent dataset, 13/61 (21%) genes were differentially expressed in lung tissues of pulmonary arterial hypertension cases versus controls, highlighting potentially novel candidate genes involved in pulmonary arterial hypertension development.

Keywords: PBMC; RNAseq; pulmonary arterial hypertension; transcriptome profiling.

Abstract

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