Coexistence and characterization of Tet(X5) and NDM-3 in the MDR-Acinetobacter indicus of duck origin

Biao Tang; Hua Yang; Xu Jia; Youjun Feng

doi:10.1016/j.micpath.2020.104697

Coexistence and characterization of Tet(X5) and NDM-3 in the MDR-Acinetobacter indicus of duck origin

Microb Pathog. 2021 Jan:150:104697. doi: 10.1016/j.micpath.2020.104697. Epub 2020 Dec 24.

Authors

Biao Tang¹, Hua Yang², Xu Jia³, Youjun Feng⁴

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, China; Department of Pathogen Biology & Microbiology and Department of General Intensive Care Unit of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, China.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, China.
³ Non-coding RNA and Drug Discovery Key Laboratory of Sichuan Province, Chengdu Medical College, Chengdu, Sichuan, 610500, China.
⁴ Department of Pathogen Biology & Microbiology and Department of General Intensive Care Unit of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, China; Non-coding RNA and Drug Discovery Key Laboratory of Sichuan Province, Chengdu Medical College, Chengdu, Sichuan, 610500, China; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, China. Electronic address: fengyj@zju.edu.cn.

PMID: 33347964
DOI: 10.1016/j.micpath.2020.104697

Abstract

Tigecycline and carbapenem are last-resort antibiotics for serious infections caused by pathogens with multi-drug resistance (MDR). Whereas, bacterial pathogens with co-resistance to tigecycline and carbapenem are poorly addressed. Here we report a tigecycline- and carbapenem-resistant Acinetobacter indicus strain HY20 of duck origin, which co-produces Tet(X5) and NDM-3. Tet(X5) is harbored by a novel plasmid pAI01 (116,992 bp long), which carries 10 antimicrobial resistance genes (AMRs), and heavy metal resistance system cobalt-zinc-cadmium (czc) gene cluster. Unlike that tet(X5) is located in the res-tet(X5)-xerD segment of plasmid, the chromosomal bla_NDM-3 is flanked by insertion ISAba125. Collectively, our result represents an example of co-carriage of tet(X5) and bla_NDM-3, heightening the importance of AMR surveillance needed in poultry production.

Keywords: Acinetobacter indicus; Carbapenem resistance; Coexistence; NDM-3; Tet(X5); Tigecycline resistance.

MeSH terms

Acinetobacter* / genetics
Animals
Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / therapeutic use
Drug Resistance, Multiple
Ducks*
Microbial Sensitivity Tests
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
beta-Lactamases

Supplementary concepts

Acinetobacter indicus