Stem cell spheroid engineering with osteoinductive and ROS scavenging nanofibers for bone regeneration

Biofabrication. 2021 Apr 2;13(3). doi: 10.1088/1758-5090/abd56c.


Stem cell spheroids have been widely investigated to accelerate bone tissue regeneration. However, the directed differentiation of stem cells into osteoblastic lineage and the prevention of cells from damage by reactive oxygen species (ROS) remain challenge. Here, we developed osteoinductive and ROS scavenging extracellular matrix-mimicking synthetic fibers based on epigallocatechin gallate (EGCG) coating. They were then utilized to fabricate engineered spheroids with human adipose-derived stem cells (hADSCs) for bone tissue regeneration. The EGCG-mineral fibers (EMF) effectively conferred osteoinductive and ROS scavenging signals on the hADSCs within spheroids, demonstrating relative upregulation of antioxidant genes (SOD-1(25.8 ± 2.1) andGPX-1(3.3 ± 0.1) and greater level of expression of osteogenic markers,runt-related transcription factor(5.8 ± 0.1) andosteopontin(5.9 ± 0.1), compared to hADSCs in the spheroids without EMF. Thein vitrooverexpression of osteogenic genes from hADSCs was achieved from absence of osteogenic supplements. Furthermore,in vivotransplantation of hADSCs spheroids with the EMF significantly promoted calvarial bone regeneration (48.39 ± 9.24%) compared to that from defect only (17.38 ± 6.63%), suggesting that the stem cell spheroid biofabrication system with our novel mineralization method described here is a promising tool for bone tissue regeneration.

Keywords: biomineralization; bone tissue engineering; epigallocatechin gallate; metal-phenolic network; simulated body fluid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue
  • Bone Regeneration
  • Cell Differentiation
  • Cells, Cultured
  • Humans
  • Nanofibers*
  • Osteogenesis
  • Reactive Oxygen Species
  • Stem Cells


  • Reactive Oxygen Species