[Role of the lncRNA H19/ microRNA-203a/PTEN axis in ischemia-reperfusion injury of mouse GC-1 cells and its mechanism]

Zhonghua Nan Ke Xue. 2020 Jun;26(6):499-504.
[Article in Chinese]


Objective: To investigate the expression of long non-coding RNA (lncRNA) H19 in mouse GC-1 cells in vitro and its effects on the proliferation and apoptosis of GC-1 cells.

Methods: We established an in vitro hypoxia-reoxygenation model in GC-1 cells and detected the expression of lncRNA H19 in the GC-1 cells at different time points of reoxygenation injury by qRT-PCR. We determined the effects of silencing lncRNA H19 on the proliferation and apoptosis of the GC-1 cells by MTT and flow cytometry, the expressions of apoptosis-related proteins Bax and caspase-3 in the GC-1 cells by Western blot, and the expressions of microRNA-203a and PTEN by qRT-PCR and Western blot, respectively.

Results: With the prolonging of the time of reoxygenation injury, the expression of lncRNA H19 was increased significantly in the GC-1 cells and peaked at 3-hour hypoxia and 12-hour reoxygenation, but that of microRNA-203a markedly decreased. Silencing lncRNA H19 enhanced the proliferation and inhibited the apoptosis of the GC-1 cells, and up-regulated the expression of microRNA-203a and down-regulated that of PTEN in the GC-1 cells.

Conclusions: LncRNA H19 is highly expressed in GC-1 cells in vitro, which may influence the proliferation and apoptosis of GC-1 cells by regulating the microRNA-203a /PTEN signaling pathway.

Keywords: GC-1 cell; PTEN; lncRNA H19; microRNA-203a; testicular ischemia-reperfusion injury.

MeSH terms

  • Animals
  • Apoptosis*
  • Cell Proliferation
  • Cells, Cultured
  • Male
  • Mice
  • MicroRNAs / genetics*
  • PTEN Phosphohydrolase / metabolism
  • RNA, Long Noncoding / genetics*
  • Reperfusion Injury* / genetics
  • Signal Transduction
  • Spermatogonia / cytology*


  • H19 long non-coding RNA
  • MIRN203 microRNA, mouse
  • MicroRNAs
  • RNA, Long Noncoding
  • PTEN Phosphohydrolase
  • Pten protein, mouse