Androstanediol glucuronide isomers in normal men and women and in men infused with labeled dihydrotestosterone

J Clin Endocrinol Metab. 1988 Jan;66(1):212-6. doi: 10.1210/jcem-66-1-212.

Abstract

3 alpha-Androstanediol glucuronide (Adiol G) is a major metabolite of dihydrotestosterone (DHT). Adiol G actually represents 2 different compounds, since the glucuronide can be conjugated at the 3-carbon position (Adiol 3-G) or at the 17-carbon position (Adiol 17-G). To determine which glucuronide represents the predominant physiological DHT metabolite and which isomer is the major circulating form, we developed a RIA to directly measure Adiol 3-G in serum extracts. In 10 normal men, mean serum Adiol 3-G and total Adiol G levels were 4.44 +/- 0.49 (+/- SE) nmol/L (208 +/- 23 ng/dL) and 27.9 +/- 2.8 nmol/L (1310 +/- 129 ng/dL), respectively (13.9 +/- 3.0% of Adiol G was Adiol 3-G). In 10 normal women sampled during the early follicular phase, mean serum Adiol 3-G and total Adiol G levels were 2.64 +/- 0.64 nmol/L (124 +/- 30 ng/dL) and 14.9 +/- 1.5 nmol/L (697 +/- 69 ng/dL), respectively (17.4 +/- 3.6% of Adiol G was Adiol 3-G). In 4 normal men infused for 8 h with tritiated DHT, 17.4 +/- 3.4% of the resulting tritiated Adiol G was Adiol 3-G. These results indicate that Adiol 17-G is the predominant circulating form of Adiol G in normal men and women and that it is also the major Adiol G isomer derived from DHT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Androstane-3,17-diol / analogs & derivatives
  • Androstane-3,17-diol / blood*
  • Androstanols / blood*
  • Chromatography, High Pressure Liquid
  • Dihydrotestosterone / blood*
  • Female
  • Humans
  • Male
  • Radioimmunoassay
  • Reference Values
  • Tritium

Substances

  • Androstanols
  • Dihydrotestosterone
  • Tritium
  • Androstane-3,17-diol
  • androstane-3,17-diol glucuronide
  • 17-hydroxyandrostane-3-glucuronide