Autoantibodies to the heat-shock protein hsp90 in systemic lupus erythematosus

J Clin Invest. 1988 Jan;81(1):106-9. doi: 10.1172/JCI113280.

Abstract

Patients with systemic lupus erythematosus (SLE) develop multiple autoantibodies to self-antigens. Analysis of autoantibody systems in this and related autoimmune disorders can provide information of etiologic and pathogenetic significance. We report here a previously unrecognized autoantibody to the 90,000-D heat-shock protein, hsp90, a molecule thought to have important functions in the cellular response to stress, virus-induced transformation, steroid hormone receptor action, and cellular activation. Autoantibodies to hsp90 were exclusively of the IgG class, and were detected in approximately 50% of unselected patients with SLE and 2/6 patients with idiopathic polymyositis. Anti-hsp90 antibodies were not detected in sera from 10 normal subjects, 10 patients with rheumatoid arthritis, or 7 patients with scleroderma. The identity of this major intracytoplasmic antigen was established by its specific removal from nonionic detergent cell lysates following immunoabsorption with monospecific rabbit anti-hsp90, and by demonstration of increased synthesis following a 10-min 45 degrees C heat shock. These data define the frequent occurrence of a novel autoantibody to a major heat-shock protein in patients with SLE.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Autoantibodies / analysis*
  • Autoantigens / analysis*
  • Autoantigens / immunology
  • Heat-Shock Proteins / immunology*
  • Humans
  • Immunoglobulin G / analysis
  • Lupus Erythematosus, Systemic / immunology*
  • Molecular Weight

Substances

  • Autoantibodies
  • Autoantigens
  • Heat-Shock Proteins
  • Immunoglobulin G