Tmprss2 specific miRNAs as promising regulators for SARS-CoV-2 entry checkpoint

Virus Res. 2021 Mar:294:198275. doi: 10.1016/j.virusres.2020.198275. Epub 2021 Jan 8.


Tmprss2 is an emerging molecular target which guides cellular infections of SARS-CoV-2, has been earmarked for interventions against the viral pathologies. The study aims to computationally screen and identifies potential miRNAs, following in vitro experimental validation of miRNA-mediated suppression of Tmprss2 for early prevention of COVID-19. Pool of 163 miRNAs, scrutinized for Tmprss2 binding with three miRNA prediction algorithms, ensued 11 common miRNAs. Further, computational negative energies for association, corroborated miRNA-Tmprss2 interactions, whereas three miRNAs (hsa-miR-214, hsa-miR-98 and hsa-miR-32) based on probability scores ≥0.8 and accessibility to Tmprss2 target have been selected in the Sfold tool. Transfection of miRNA(s) in the Caco-2 cells, quantitatively estimated differential expression, confirming silencing of Tmprss2 with maximum gene suppression by hsa-miR-32 employing novel promising role in CoV-2 pathogenesis. The exalted binding of miRNAs to Tmprss2 and suppression of later advocates their utility as molecular tools for prevention of SARS-CoV-2 viral transmission and replication in humans.

Keywords: COVID-19; Tmprss2; hsa-miR-214; hsa-miR-32; hsa-miR-98.

MeSH terms

  • Caco-2 Cells
  • Computational Biology
  • Computer Simulation
  • Gene Silencing
  • Humans
  • MicroRNAs / chemistry
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Nucleic Acid Conformation
  • SARS-CoV-2 / physiology*
  • Serine Endopeptidases / genetics*
  • Virus Internalization*


  • MicroRNAs
  • Serine Endopeptidases
  • TMPRSS2 protein, human