Increased Endocannabinoid Signaling Reduces Social Motivation in Intact Rats and Does Not Affect Animals Submitted to Early-Life Seizures

Fernanda Teixeira Ribeiro; Marcia Ivany Silva de Serro-Azul; Fernanda Beraldo Lorena; Bruna Pascarelli Pedrico do Nascimento; Alexandre José Tavolari Arnold; Geraldo Henrique Lemos Barbosa; Miriam Oliveira Ribeiro; Roberta Monterazzo Cysneiros

doi:10.3389/fnbeh.2020.560423

Increased Endocannabinoid Signaling Reduces Social Motivation in Intact Rats and Does Not Affect Animals Submitted to Early-Life Seizures

Front Behav Neurosci. 2020 Dec 9:14:560423. doi: 10.3389/fnbeh.2020.560423. eCollection 2020.

Authors

Fernanda Teixeira Ribeiro¹, Marcia Ivany Silva de Serro-Azul¹, Fernanda Beraldo Lorena², Bruna Pascarelli Pedrico do Nascimento², Alexandre José Tavolari Arnold¹, Geraldo Henrique Lemos Barbosa¹, Miriam Oliveira Ribeiro¹, Roberta Monterazzo Cysneiros¹

Affiliations

¹ Developmental Disabilities Postgraduate Program, Laboratory of Neurobiology and Metabolism, Mackenzie Presbyterian University, São Paulo, Brazil.
² Postgraduate Program in Translational Medicine, Federal University of São Paulo, São Paulo, Brazil.

Abstract

The early life status epilepticus (SE) causes high anxiety and chronic socialization abnormalities, revealed by a low preference for social novelty and deficit in social discrimination. This study investigated the involvement of the endocannabinoid system on the sociability in this model, due to its role in social motivation regulation. Male Wistar rats at postnatal day 9 were subjected to pilocarpine-induced neonatal SE and controls received saline. From P60 the groups received vehicle or JZL195 2 h before each behavioral test to increase endocannabinoids availability. In the sociability test, animals subjected to neonatal SE exhibited impaired sociability, characterized by social discrimination deficit, which was unaffected by the JZL195 treatment. In contrast, JZL195-treated control rats showed low sociability and impaired social discrimination. The negative impact of JZL195 over the sociability in control rats and the lack of effect in animals subjected to neonatal SE was confirmed in the social memory paradigm. In this paradigm, as expected for vehicle-treated control rats, the investigation toward the same social stimulus decreased with the sequential exposition and increased toward a novel stimulus. In animals subjected to neonatal SE, regardless of the treatment, as well as in JZL195-treated control rats, the investigation toward the same social stimulus was significantly reduced with no improvement toward a novel stimulus. Concerning the locomotion, the JZL195 increased it only in control rats. After behavioral tests, brain tissues of untreated animals were used for CB1 receptor quantification by Elisa and for gene expression by RT-PCR: no difference between control and experimental animals was noticed. The results reinforce the evidence that the early SE causes chronic socialization abnormalities, revealed by the low social interest for novelty and impaired social discrimination. The dual FAAH/MAGL inhibitor (JZL195) administration before the social encounter impaired the social interaction in intact rats with no effect in animals subjected to early-life seizures.

Keywords: CB1 receptor; JZL195; autism (ASD); endocannabinoid system; pilocarpine; seizures; sociability; social reward processing.