Profiling of mouse macrophage lipidome using direct infusion shotgun mass spectrometry

STAR Protoc. 2020 Dec 19;2(1):100235. doi: 10.1016/j.xpro.2020.100235. eCollection 2021 Mar 19.


Immune cells, such as macrophages, reprogram their lipid metabolism in response to the activation of pattern recognition receptors (e.g., TLRs, NLRs) and cytokine receptors (e.g., interferons, interleukins). Profiling these changes can be achieved with shotgun mass spectrometry. This protocol provides step-by-step instructions on the generation and stimulation of bone marrow-derived macrophages (BMDMs), sample collection, and lipid extraction for profiling the macrophage lipidome. For complete details on the use and execution of this protocol, please refer to Hsieh et al. (2020).

Keywords: Cell culture; High Throughput Screening; Immunology; Mass Spectrometry; Metabolism; Model Organisms.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Lipid Metabolism*
  • Lipidomics*
  • Macrophages / metabolism*
  • Mass Spectrometry*
  • Mice