Phylogenetic analysis and characterization of the complete chloroplast genome of Dipsacus asperoides, the endemic medicinal herb in China

Tianyi Cao; Jun Fei; Gang Zu; Guihe Han; Zhen Lai; Ning Ren; Quan Zhang

doi:10.1080/23802359.2019.1640648

Phylogenetic analysis and characterization of the complete chloroplast genome of Dipsacus asperoides, the endemic medicinal herb in China

Mitochondrial DNA B Resour. 2019 Jul 18;4(2):2557-2559. doi: 10.1080/23802359.2019.1640648.

Authors

Tianyi Cao¹, Jun Fei², Gang Zu², Guihe Han², Zhen Lai², Ning Ren³, Quan Zhang²

Affiliations

¹ Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
² Department of Orthopaedics, Zhejiang Integrated Traditional Chinese and Western Medicine Hospital, Hangzhou, Zhejiang, China.
³ Hangzhou Xiaoshan Ruan Health Management Co., Ltd, Hangzhou, Zhejiang, China.

Abstract

Dipsacus asperoides is a traditional and endemic medicinal herb in China. Its roots was considered top grade herb as early as in the Shen Nong Herbal Classic. In this study, we assembled and analyzed the complete chloroplast genome of D. asperoides. The complete chloroplast genome is 160,481 bp in length, exhibiting a large single-copy region (88,546 bp), a small single-copy region (19,671 bp), and two inverted-repeat regions (26,132 bp in each one). The chloroplast genome of D. asperoides contains 133 genes, including 89 protein-coding genes (PCGs), 36 transfer RNA (tRNAs), and eight ribosome RNA (rRNAs). The overall nucleotide content of the chloroplast genome is A of 30.2%, T of 31.0%, C of 19.7%, and G of 19.1%, with a total AT content of 61.2% and GC content of 38.8%. However, the phylogenetic Maximum-Likelihood (ML) analysis based on the amino acid sequences of 89 PCGs from 14 species chloroplast genome that D. asperoides is closely related to Dipsacus asper. This study can be used for medicinal herb value research and clinical drug development.

Keywords: Dipsacaceae; Dipsacus asperoides; chloroplast genome; medicinal herb; phylogenetic analysis.