Single-molecule sequencing yields the complete chloroplast genome sequence of Populus deltoides

Dongyang Wu; Yupeng Wang; Donghua Wu; Lijun Dou; Linming Gao

doi:10.1080/23802359.2019.1698346

Single-molecule sequencing yields the complete chloroplast genome sequence of Populus deltoides

Mitochondrial DNA B Resour. 2019 Dec 12;5(1):125-126. doi: 10.1080/23802359.2019.1698346.

Authors

Dongyang Wu¹, Yupeng Wang¹, Donghua Wu², Lijun Dou³, Linming Gao⁴

Affiliations

¹ College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.
² College of Continuing Education, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, China.
³ Information Center, Nanjing Forestry University, Nanjing, Jiangsu, China.
⁴ Computer Center of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.

Abstract

Populus deltoides is a fast-growing, large tree and one of the largest North American hardwood trees. In this study, the complete chloroplast (cp) genome sequence of P. deltoides is characterized. The whole cp genome was assembled to 156,867 bp, including a large single copy (LSC) region of 85,534 bp, a small single copy (SSC) region of 16,513 bp and a pair of inverted repeats (IRs) region of 27,410 bp. The base content of the P. deltoides cp genome is A (32.0%), T (31.3%), C (18.0%), and G (18.7%), and AT bases occupy a large proportion of the cp genome. The neighbor-joining phylogenetic analysis with 20 cp genomes from the Salicaceae family showed that P. deltoides is sister to Populus davidiana. These will provide for the evolutionary and biological studies in Salicaceae family.

Keywords: Populus deltoides; chloroplast genome; phylogeny.

Grants and funding

National Key Research and Development Plan of China.