MicroRNA-2355-5p regulates γ-globin expression in human erythroid cells by inhibiting KLF6

Br J Haematol. 2021 Apr;193(2):401-405. doi: 10.1111/bjh.17134. Epub 2020 Dec 24.

Abstract

Krüppel-like factors (KLFs) are a highly conserved family of transcription factors. We analysed expression profile data of KLFs and identified KLF6 as a new potential regulator of erythropoiesis. Knocking down the expression of KLF6 significantly raised γ-globin mRNA and protein levels in the erythroid cell line HUDEP-2 and haematopoietic progenitor (CD34+ ) cells. We found that overexpression of microRNA (miR)-2355-5p in HUDEP-2 and CD34+ cells correlated with increased γ-globin synthesis by suppressing expression of KLF6. Our discovery that the interaction between miR-2355-5p and KLF6 affects the expression of γ-globin may provide more information for the clinical management of β-thalassaemia patients.

Keywords: HbF; KLF6; miR-2355-5p; β-thalassaemia; γ-globin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34 / metabolism
  • Cell Differentiation / genetics
  • Erythroid Cells / metabolism*
  • Erythropoiesis / genetics
  • Fetal Hemoglobin / genetics*
  • Humans
  • Kruppel-Like Factor 6 / genetics
  • Kruppel-Like Factor 6 / pharmacology
  • Kruppel-Like Transcription Factors / genetics
  • MicroRNAs / genetics*
  • Transcription Factors / genetics
  • beta-Thalassemia / genetics
  • beta-Thalassemia / therapy
  • gamma-Globins / genetics*

Substances

  • Antigens, CD34
  • KLF6 protein, human
  • Kruppel-Like Factor 6
  • Kruppel-Like Transcription Factors
  • MIRN2355 microRNA, human
  • MicroRNAs
  • Transcription Factors
  • gamma-Globins
  • Fetal Hemoglobin