Herein, we investigated the antioxidant and hepatoprotective effects of thyme (Thymus munbyanus: AETM) on 2,4-dichlorophenoxyacetic acid (2,4 -D) - induced liver oxidative damage in rats. The phytochemical study of AETM revealed potent antioxidant properties owed to its richness in phenolic compounds including flavonoids, tannins, and phenolic acids. Further, in vivo animal study was conducted on 24 Wistar rats divided equally into control group and three treated groups, receiving orally AETM (10 ml/kg body weight (b.w), 2,4-D (5 mg/kg (b.w) and AETM + 2,4 - D (combined treatment) for 30 consecutive days. The results showed a significant increase in the enzymatic activity of transaminases (AST, ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (γ-GT), lactate dehydrogenase (LDH), and the levels of malondialdehyde (MDA) and carbonyl proteins (CPO), along with a significant decrease in plasma total protein, albumin, hepatic glutathione (GSH) contents, and the enzymatic activity of the hepatic antioxidant markers (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione- S- transferase (GST)) in 2,4- D treatment compared with control. Moreover, no significant changes in these parameters were noticed in AETM treated animals as compared to control, and hence the combined treatment (AETM + 2,4- D) showed a marked enhancement in the above altered hepatic functional and antioxidant parameters and liver histopathology. In conclusion, AETM, owing to its richness with phenolic compounds proved to be an efficient antioxidant against 2,4-D - induced liver oxidative damage, and hence complementary studies would be needed to appear the use of these compounds as supplements in treating liver impairment.
Keywords: 2,4-D; Thymus munbyanus; antioxidant; liver injury; oxidative stress; rat.