Detailed analysis of the fatty acid composition of six plant-pathogenic bacteria

Nina Wiedmaier-Czerny; Dorothee Schroth; Shiri Topman-Rakover; Aya Brill; Saul Burdman; Zvi Hayouka; Walter Vetter

doi:10.1016/j.jchromb.2020.122454

Detailed analysis of the fatty acid composition of six plant-pathogenic bacteria

J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Jan 1:1162:122454. doi: 10.1016/j.jchromb.2020.122454. Epub 2020 Dec 3.

Authors

Nina Wiedmaier-Czerny¹, Dorothee Schroth¹, Shiri Topman-Rakover², Aya Brill², Saul Burdman³, Zvi Hayouka⁴, Walter Vetter⁵

Affiliations

¹ Institute of Food Chemistry, Department of Food Chemistry (170b), University of Hohenheim, D-70593 Stuttgart, Germany.
² Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel; Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
³ Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
⁴ Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel. Electronic address: zvi.hayouka@mail.huji.ac.il.
⁵ Institute of Food Chemistry, Department of Food Chemistry (170b), University of Hohenheim, D-70593 Stuttgart, Germany. Electronic address: walter.vetter@uni-hohenheim.de.

PMID: 33373896
DOI: 10.1016/j.jchromb.2020.122454

Abstract

Bacteria show distinct and characteristic fatty acid (FA) patterns which can be modified by environmental conditions. In this study, we cultivated six plant-pathogenic bacteria of agricultural concern and performed a detailed analysis of the fatty acid composition. The study covered four strains of the gram-negative Xanthomonas campestris pathovar (pv) campestris (Xcc), Xanthomonas perforans (Xp), Acidovorax citrulli (Ac) and Pseudomonas syringae pv. tomato (Pst), and two strains of the gram-positive Clavibacter michiganensis subsp. michiganensis (Cmm) and Streptomyces scabies (Ssc). After cultivation, freeze-dried bacteria samples were transesterified and analysed by gas chromatography with mass spectrometry in full scan and selected ion monitoring (SIM) modes. Altogether, 44 different FAs were detected in the six strains with individual contributions of 0.01-43.8% to the total FAs. The variety in the six strains ranged between 12 and 31 individual FAs. The FA composition of Xcc, Xp, Cmm and Ssc were dominated by iso- and anteiso-fatty acids (especially i15:0, a15:0, i16:0), which is typical for most bacteria. In contrast to this, Ac and Pst showed only saturated and monounsaturated FAs. Four of the six bacteria showed similar FA patterns as reported before in the literature. Differences were observed in the case of Cmm where many more FAs were detected in the present study. In addition, to the best of our knowledge, the FA pattern of Xp was presented for the first time.

Keywords: A. citrulli; C. michiganensis; Fatty acid pattern; Iso-/anteiso-fatty acid; P. syringae; Plant-pathogenic bacteria; S. scabies; X. campestris; X. perforans.

MeSH terms

Bacteria / chemistry*
Bacteria / pathogenicity
Crops, Agricultural / microbiology*
Fatty Acids / analysis*
Fatty Acids / chemistry
Limit of Detection
Linear Models
Plant Diseases / microbiology*
Reproducibility of Results

Substances

Fatty Acids