Efficacy of Sea Salt-Based Mouthwash and Xylitol in Improving Oral Hygiene among Adolescent Population: A Pilot Study

Int J Environ Res Public Health. 2020 Dec 23;18(1):44. doi: 10.3390/ijerph18010044.


The scientific community has definitely demonstrated the importance of the use of mouthwash in daily oral hygiene. In our pilot study, we tested the effectiveness of a novel mouth rinse containing sea salt, xylitol, and lysozyme. Streptococcus mutans (S. mutans) growth, and plaque index in adolescent patients aged 14-17 years, were observed. The bacterial load was investigated by in vitro microbiological analysis; the plaque index was assessed through the O'Leary's Plaque Control Record (PCR). The study has shown that the use of a sea salt-based mouthwash in daily oral hygiene reduces the bacterial levels of S. mutans (p < 0.01) linked to the combined action of xylitol and lysozyme, together with the action of sea salt. Our preliminary data confirm and improve the main results reported in the scientific literature on the importance of the use of xylitol, lysozyme, and sea salt in oral health.

Keywords: Streptococcus mutans; clinical microbiology; lysozyme; oral health; plaque index; sea salt; xylitol.

MeSH terms

  • Adolescent
  • Dental Plaque / prevention & control
  • Humans
  • Mouthwashes / therapeutic use*
  • Muramidase / therapeutic use
  • Oral Hygiene*
  • Pilot Projects
  • Sodium Chloride / therapeutic use*
  • Streptococcus mutans
  • Xylitol / therapeutic use*


  • Mouthwashes
  • Sodium Chloride
  • Muramidase
  • Xylitol