Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets

Aymeric Chorlay; Alexandre Santinho; Abdou Rachid Thiam

doi:10.1016/j.xpro.2020.100116

Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets

STAR Protoc. 2020 Sep 25;1(3):100116. doi: 10.1016/j.xpro.2020.100116. eCollection 2020 Dec 18.

Authors

Aymeric Chorlay¹, Alexandre Santinho¹, Abdou Rachid Thiam¹

Affiliation

¹ Laboratoire de Physique de l'École Normale Supérieure, ENS, Université PSL, CNRS, Sorbonne Université, Université de Paris, 75005 Paris, France.

Abstract

We present a reproducible protocol to prepare droplet-embedded vesicles (DEVs) consisting of an oil droplet embedded within a phospholipid bilayer. This model system mimics a cellular lipid droplet (LD) in physical contact with the endoplasmic reticulum (ER) bilayer. It has the advantage that the lipid composition and the biophysical properties of the droplet and the bilayer are controlled and tunable. DEVs can be used to study LD biogenesis factors and determinants of protein binding between ER and LD interfaces. For complete details on the use and execution of this protocol, please refer to Chorlay and Thiam (2020) and Santinho et al. (2020).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Lipid Droplets / chemistry*
Lipid Droplets / metabolism*
Lipid Droplets / physiology*
Lipid Metabolism
Membrane Proteins / metabolism
Models, Biological
Phospholipids / metabolism

Substances

Membrane Proteins
Phospholipids