Generation of Casp8FL122/123GG Mice Using CRISPR-Cas9 Technology

STAR Protoc. 2020 Nov 25;1(3):100181. doi: 10.1016/j.xpro.2020.100181. eCollection 2020 Dec 18.

Abstract

The purpose of this protocol is to describe the generation of missense mutations in mice using CRISPR-Cas9 technology. The current protocol focuses on the generation of a Casp8FL122/123GG missense mutation, but it can be adapted to introduce any missense or nonsense mutation. For complete details on the use and execution of this protocol, please refer to Tummers et al. (2020).

Keywords: CRISPR; Model Organisms.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • CRISPR-Associated Protein 9 / genetics
  • CRISPR-Associated Protein 9 / metabolism*
  • CRISPR-Cas Systems / genetics*
  • Caspase 8 / genetics*
  • Female
  • Genotype
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microinjections
  • Polymerase Chain Reaction
  • RNA, Guide, Kinetoplastida / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Recombinant Proteins / metabolism
  • Zygote / metabolism

Substances

  • RNA, Guide
  • RNA, Messenger
  • Recombinant Proteins
  • CRISPR-Associated Protein 9
  • Caspase 8