Obtaining patient-derived cancer organoid cultures via fine-needle aspiration

STAR Protoc. 2020 Dec 21;2(1):100220. doi: 10.1016/j.xpro.2020.100220. eCollection 2021 Mar 19.


Patient-derived tumor organoid cultures are an essential and innovative methodology for translational research. However, current techniques to establish these cultures are cumbersome, expensive, and often require irreplaceable clinical tissue from surgery or core biopsies. Fine-needle aspiration (FNA) provides a minimally invasive biopsy technique commonly performed in clinical settings. Here, we provide a protocol for FNA. We have found that FNA provides a cost-effective, rapid, and streamlined method for tissue acquisition for cancer organoid culture. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020).

Keywords: cancer; cell culture; cell isolation; organoids.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biopsy, Fine-Needle
  • Female
  • Humans
  • Male
  • Neoplasms* / metabolism
  • Neoplasms* / pathology
  • Organoids* / metabolism
  • Organoids* / pathology
  • Tumor Cells, Cultured