Biosynthesis of monoterpene hydrocarbons by isolated chromoplasts from daffodil flowers

Eur J Biochem. 1988 Jan 4;170(3):613-6. doi: 10.1111/j.1432-1033.1988.tb13741.x.

Abstract

Incubation studies using [1-14C]isopentenyl diphosphate and [1-2H2]isopentenyl diphosphate as substrates revealed that isolated chromoplasts from flowers of Narcissus pseudonarcissus L. are able to synthesize monoterpene hydrocarbons and linalool in high yields. The enzymes involved are soluble in the chromoplast stroma. It is hypothesized that in the plant cell plastids are the site of monoterpene biosynthesis, whereas the formation of sesquiterpenes may be restricted to the cytoplasm/endoplasmic reticulum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Radioisotopes
  • Hemiterpenes*
  • Mass Spectrometry
  • Organoids / metabolism*
  • Organophosphorus Compounds / metabolism
  • Plants / metabolism*
  • Terpenes / metabolism*
  • Tritium

Substances

  • Carbon Radioisotopes
  • Hemiterpenes
  • Organophosphorus Compounds
  • Terpenes
  • Tritium
  • isopentenyl pyrophosphate