Salivary pellicle modification with polyphenol-rich teas and natural extracts to improve protection against dental erosion

Samira Helena Niemeyer; Tommy Baumann; Adrian Lussi; Hendrik Meyer-Lueckel; Taís Scaramucci; Thiago Saads Carvalho

doi:10.1016/j.jdent.2020.103567

Salivary pellicle modification with polyphenol-rich teas and natural extracts to improve protection against dental erosion

J Dent. 2021 Feb:105:103567. doi: 10.1016/j.jdent.2020.103567. Epub 2020 Dec 30.

Authors

Samira Helena Niemeyer¹, Tommy Baumann², Adrian Lussi³, Hendrik Meyer-Lueckel⁴, Taís Scaramucci⁵, Thiago Saads Carvalho⁶

Affiliations

¹ Department of Restorative, Preventive and Pediatric Dentistry, University of Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland. Electronic address: samira.joaodesouza@zmk.unibe.ch.
² Department of Restorative, Preventive and Pediatric Dentistry, University of Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland. Electronic address: tommy.baumann@zmk.unibe.ch.
³ Department of Restorative, Preventive and Pediatric Dentistry, University of Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland. Electronic address: adrian.lussi@zmk.unibe.ch.
⁴ Department of Restorative, Preventive and Pediatric Dentistry, University of Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland. Electronic address: hendrik.meyer-lueckel@zmk.unibe.ch.
⁵ Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, Cidade Universitária, São Paulo, SP, Zip code: 05508-000, Brazil. Electronic address: tais.sca@usp.br.
⁶ Department of Restorative, Preventive and Pediatric Dentistry, University of Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland. Electronic address: thiago.saads@zmk.unibe.ch.

PMID: 33387569
DOI: 10.1016/j.jdent.2020.103567

Abstract

Objective: To investigate the modification of the salivary pellicle with different polyphenol-rich teas and natural extracts for the protection against dental erosion.

Methods: We performed two experiments: one with teas (Green tea, Black tea, Peppermint tea, Rosehip tea, negative control [NC]) and other with natural extracts (Grape seed, Grapefruit seed, Cranberry, Propolis, NC), where NC was deionized water. A total of 150 enamel specimens were used (n = 15/group). Both experiments followed the same design, consisting of 5 cycles of: salivary pellicle formation (30 min, 37 °C), modification with the solutions (30 min, 25 °C), further salivary pellicle formation (60 min, 37 °C) and erosive challenge (1 min, 1% citric acid, pH 3.6). Relative surface microhardness (rSMH), relative surface reflection intensity (rSRI) and amount of calcium release (CaR) were evaluated. Data were analysed with Kruskal-Wallis and Wilcoxon rank sum tests with Bonferroni correction (α = 0.05).

Results: Regarding teas, Black and Green teas showed the best protection against dental erosion, presenting higher rSMH and lower CaR than NC. Peppermint tea was not different to NC and Rosehip tea caused erosion, showing the highest CaR and greatest loss of SMH and SRI. Regarding natural extracts, Grape seed and Grapefruit seed extracts presented the best protective effect, with significantly higher rSMH and lower CaR. Cranberry caused significantly more demineralization; and Propolis did not differ from NC.

Conclusion: Green tea, Black tea, Grape seed extract and Grapefruit seed extract were able to modify the salivary pellicle and improve its protective effect against enamel erosion, but Rosehip tea and Cranberry extract caused erosion.

Clinical relevance: Some some bio-products, such as teas and natural extracts, improve the protective effect of the salivary pellicle against enamel erosion. More studies should be performed in order to test the viability of their use as active ingredients for oral care products.

Keywords: Acquired salivary pellicle; Dental erosion; Enamel; Polyphenols; Tea.

MeSH terms

Dental Enamel
Dental Pellicle
Humans
Plant Extracts / therapeutic use
Polyphenols
Tooth Erosion* / prevention & control

Substances

Plant Extracts
Polyphenols