Taking banana continuous planting soil with high banana fusarium wilt disease incidence as a test site, we examined the effect of lime and ammonium carbonate fumigation coupled with bio-organic fertilizer on the suppression of banana fusarium wilt disease and the structure and composition of bacterial community, using real-time quantitative PCR and high-throughput sequencing. The results showed that the disease incidence was reduced by 13.3% and 21.7% in the treatments of LAOF (lime and ammonium carbonate fumigation coupled with organic fertilizer) and LABF (lime and ammonium carbonate fumigation coupled with bio-organic fertilizer), respectively, compared with OF (application of organic fertilizer without fumigation), while the copy number of Fusarium was decreased by 22.4% and 33.0%, respectively. Compared with non-fumigation treatment, lime and ammonium fumigation coupled with different fertilizer applications significantly reduced bacteria richness and diversity, with different community structure, while fumigation had a decisive effect on bacterial community composition. Bacterial richness and diversity of LABF were lower than those of other treatments, while microbial community structure was clearly disparate from other treatments. Compared with non-fumigation treatment, the relative abundance of Mizugakiibacter, Brucella, and Rhodanobacter were significantly improved in the fumigation coupled with different fertilization treatments. Those three genera in LABF were higher than those in LAOF, with significant differences for the relative abundances of Mizugakiibacter and Brucella. Therefore, fumigation combined with bio-organic fertilizer application could reduce the copy number of pathogen, alter soil bacterial community structure and stimulate beneficial bacteria in the resident soil, and thus reduce the occurrence of banana fusarium wilt.
以连续种植的香蕉枯萎病高发病蕉园为试验点,通过实时定量PCR和高通量测序等方法,研究了田间条件下石灰碳铵熏蒸联合生物有机肥施用对香蕉枯萎病的防治效果,以及对土壤细菌群落结构和组成的影响。结果表明: 与不熏蒸施用有机肥(OF)相比,香蕉枯萎病发病率在施用有机肥前使用石灰碳铵进行熏蒸处理(LAOF)和施用生物有机肥前使用石灰碳铵进行熏蒸处理(LABF)中分别降低了13.3%和21.7%,病原菌的拷贝数分别降低了22.4%和33.0%。与OF处理相比,石灰碳铵熏蒸联合不同肥料施用均显著降低了细菌的丰富度和多样性,形成了明显不同的群落结构,且熏蒸对群落组成的差异产生了决定性的影响。LABF的细菌丰富度和多样性均低于其他处理,群落组成也与其他处理存在明显差异。与OF处理相比,熏蒸处理(LAOF和LABF)显著增加了土壤中水恒杆菌、布鲁式菌和漯河杆菌属的相对丰度,且在LABF中的相对丰度均高于LAOF,水恒杆菌和布鲁式菌的相对丰度差异显著。在田间条件下,施用生物有机肥之前使用石灰碳铵进行熏蒸处理能够显著降低病原菌数量,改变土壤细菌群落结构,激发土壤有益微生物,从而减少香蕉枯萎病的发生。.
Keywords: bacterial community; banana fusarium wilt; bio-fertilizer; high-throughput sequencing; soil fumigation.