The purification of four respiratory syncytial virus proteins and their evaluation as protective agents against experimental infection in BALB/c mice

J Gen Virol. 1988 Feb:69 ( Pt 2):293-303. doi: 10.1099/0022-1317-69-2-293.

Abstract

The fusion (F) glycoprotein, large glyco- (G) protein, phospho- (P) protein and 22K protein of respiratory syncytial (RS) virus A2 strain were purified by a combination of immunoaffinity adsorption and preparative SDS-PAGE. All four proteins elicited serum antibody in mice after repeated inoculation in adjuvant, although the magnitude of the response as measured by ELISA varied from mouse to mouse. The F protein generated neutralizing antibodies in only 50% of the mice determined to be seropositive by ELISA. The G protein also induced neutralizing antibodies but in this instance neutralization tests and ELISA titres were more closely correlated. No neutralizing activity was detected in mice immunized with the P or 22K proteins although all produced antibody detectable by ELISA. Mice immunized with either the F or the G protein were found to be protected against subsequent RS virus challenge, whether they had developed neutralizing antibody or not. Mice inoculated with the P or 22K proteins were not protected.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / biosynthesis
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Glycoproteins / immunology
  • Glycoproteins / isolation & purification
  • Mice
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Phosphoproteins / immunology
  • Phosphoproteins / isolation & purification
  • Respiratory Syncytial Viruses / analysis*
  • Respiratory Syncytial Viruses / immunology
  • Respirovirus Infections / prevention & control*
  • Viral Envelope Proteins / immunology
  • Viral Envelope Proteins / isolation & purification
  • Viral Fusion Proteins / immunology
  • Viral Fusion Proteins / isolation & purification
  • Viral Proteins / immunology
  • Viral Proteins / isolation & purification*
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Viral
  • Glycoproteins
  • Phosphoproteins
  • Viral Envelope Proteins
  • Viral Fusion Proteins
  • Viral Proteins
  • Viral Vaccines