Genetic Association of a Gain-of-Function IFNGR1 Polymorphism and the Intergenic Region LNCAROD/DKK1 With Behçet's Disease

Arthritis Rheumatol. 2021 Jul;73(7):1244-1252. doi: 10.1002/art.41637. Epub 2021 May 18.

Abstract

Objective: Behçet's disease is a complex systemic inflammatory vasculitis of incompletely understood etiology. This study was undertaken to investigate genetic associations with Behçet's disease in a diverse multiethnic population.

Methods: A total of 9,444 patients and controls from 7 different populations were included in this study. Genotyping was performed using an Infinium ImmunoArray-24 v.1.0 or v.2.0 BeadChip. Analysis of expression data from stimulated monocytes, and epigenetic and chromatin interaction analyses were performed.

Results: We identified 2 novel genetic susceptibility loci for Behçet's disease, including a risk locus in IFNGR1 (rs4896243) (odds ratio [OR] 1.25; P = 2.42 × 10-9 ) and within the intergenic region LNCAROD/DKK1 (rs1660760) (OR 0.78; P = 2.75 × 10-8 ). The risk variants in IFNGR1 significantly increased IFNGR1 messenger RNA expression in lipopolysaccharide-stimulated monocytes. In addition, our results replicated the association (P < 5 × 10-8 ) of 6 previously identified susceptibility loci in Behçet's disease: IL10, IL23R, IL12A-AS1, CCR3, ADO, and LACC1, reinforcing the notion that these loci are strong genetic factors in Behçet's disease shared across ancestries. We also identified >30 genetic susceptibility loci with a suggestive level of association (P < 5 × 10-5 ), which will require replication. Finally, functional annotation of genetic susceptibility loci in Behçet's disease revealed their possible regulatory roles and suggested potential causal genes and molecular mechanisms that could be further investigated.

Conclusion: We performed the largest genetic association study in Behçet's disease to date. Our findings reveal novel putative functional variants associated with the disease and replicate and extend the genetic associations in other loci across multiple ancestries.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Behcet Syndrome / genetics*
  • Behcet Syndrome / immunology
  • Case-Control Studies
  • Chromosomes, Human, Pair 10 / genetics
  • DNA, Intergenic / genetics
  • Epigenesis, Genetic
  • Female
  • Gain of Function Mutation
  • Gene Expression Regulation
  • Genetic Predisposition to Disease
  • Humans
  • Intercellular Signaling Peptides and Proteins / genetics
  • Lipopolysaccharides
  • Male
  • Monocytes / immunology*
  • Polymorphism, Single Nucleotide
  • RNA, Long Noncoding / genetics
  • RNA, Messenger / metabolism
  • Receptors, Interferon / genetics*
  • Receptors, Interferon / immunology

Substances

  • DKK1 protein, human
  • DNA, Intergenic
  • Intercellular Signaling Peptides and Proteins
  • Lipopolysaccharides
  • RNA, Long Noncoding
  • RNA, Messenger
  • Receptors, Interferon
  • interferon gamma receptor