Self-assembled mRNA vaccines

Adv Drug Deliv Rev. 2021 Mar:170:83-112. doi: 10.1016/j.addr.2020.12.014. Epub 2021 Jan 2.

Abstract

mRNA vaccines have evolved from being a mere curiosity to emerging as COVID-19 vaccine front-runners. Recent advancements in the field of RNA technology, vaccinology, and nanotechnology have generated interest in delivering safe and effective mRNA therapeutics. In this review, we discuss design and self-assembly of mRNA vaccines. Self-assembly, a spontaneous organization of individual molecules, allows for design of nanoparticles with customizable properties. We highlight the materials commonly utilized to deliver mRNA, their physicochemical characteristics, and other relevant considerations, such as mRNA optimization, routes of administration, cellular fate, and immune activation, that are important for successful mRNA vaccination. We also examine the COVID-19 mRNA vaccines currently in clinical trials. mRNA vaccines are ready for the clinic, showing tremendous promise in the COVID-19 vaccine race, and have pushed the boundaries of gene therapy.

Keywords: COVID-19; Gene delivery; Immunization; Lipid nanoparticles; Self-assembly; mRNA delivery.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • COVID-19 Vaccines / administration & dosage*
  • COVID-19 Vaccines / genetics
  • COVID-19 Vaccines / immunology
  • Drug Design*
  • Gene Transfer Techniques* / trends
  • Humans
  • Nanoparticles / administration & dosage
  • Nanotechnology / methods*
  • Nanotechnology / trends
  • RNA, Messenger / administration & dosage*
  • RNA, Messenger / genetics
  • RNA, Messenger / immunology
  • Vaccines, Synthetic / administration & dosage*
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology
  • mRNA Vaccines

Substances

  • COVID-19 Vaccines
  • RNA, Messenger
  • Vaccines, Synthetic