Bioengineered Braided Micro-Nano (Multiscale) Fibrous Scaffolds for Tendon Reconstruction

Anjana Jayasree; Shalumon Kottappally Thankappan; Retheesh Ramachandran; M Nivedhitha Sundaram; Chih-Hao Chen; Ullas Mony; Jyh-Ping Chen; Rangasamy Jayakumar

doi:10.1021/acsbiomaterials.8b01328

Bioengineered Braided Micro-Nano (Multiscale) Fibrous Scaffolds for Tendon Reconstruction

ACS Biomater Sci Eng. 2019 Mar 11;5(3):1476-1486. doi: 10.1021/acsbiomaterials.8b01328. Epub 2019 Feb 27.

Authors

Anjana Jayasree¹, Shalumon Kottappally Thankappan², Retheesh Ramachandran², M Nivedhitha Sundaram¹, Chih-Hao Chen³, Ullas Mony¹, Jyh-Ping Chen^{2

3

4

5}, Rangasamy Jayakumar¹

Affiliations

¹ Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682 041, India.
² Department of Chemical and Materials Engineering, Chang Gung University, Kwei-San, Taoyuan 33302, Taiwan.
³ Department of Plastic and Reconstructive Surgery and Craniofacial Research Center, Chang Gung Memorial Hospital, Linkou, Chang Gung University School of Medicine, Kwei-San, Taoyuan 33305, Taiwan.
⁴ Research Center for Food and Cosmetic Safety, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33302, Taiwan.
⁵ Department of Materials Engineering, Ming Chi University of Technology, Tai-Shan, New Taipei City 24301, Taiwan.

PMID: 33405622
DOI: 10.1021/acsbiomaterials.8b01328

Abstract

A braided multiscale fibrous scaffold consisting of aligned PCL micro/collagen-bFGFnano fibers was fabricated (mPCL-nCol-bFGF) to mimic native tendon tissue architecture which was further coated with alginate to aid in prevention of peritendinous adhesion. The bFGF release kinetics showed a sustained release of growth factors for a period of 20 days. Further, in vitro cell viability, attachment, and proliferation were performed using rabbit tenocytes under static and dynamic conditions. mPCL-nCol-bFGF showed a higher cell proliferation and enhanced expression of tenogenic markers compared to mPCL-nCol (braided scaffold without bFGF). When subjected to dynamic stimulation in a bioreactor, mPCL-nCol-bFGF-DS (braided scaffold with bFGF after dynamic stimulation) showed enhanced cellular proliferation and tenogenic marker expression, compared to mPCL-nCol-bFGF. The in vivo studies of the cell seeded scaffold after dynamic stimulation in Achilles tendon defect model showed tendon tissue regeneration with aligned collagen morphology within 12 weeks of implantation.

Keywords: bFGF; braided scaffold; multiscale fibers; poly(ε-caprolactone); tendon regeneration.