Extended-Spectrum β-Lactamases in Human Isolates of Multidrug-Resistant Non-typhoidal Salmonella enterica

Anahit M Sedrakyan; Zhanna A Ktsoyan; Karine A Arakelova; Magdalina K Zakharyan; Alvard I Hovhannisyan; Zaruhi U Gevorgyan; Armine A Mnatsakanyan; Elene G Kakabadze; Khatuna B Makalatia; Nina A Chanishvili; Jean-Paul Pirnay; Arsen A Arakelyan; Rustam I Aminov

doi:10.3389/fmicb.2020.592223

Extended-Spectrum β-Lactamases in Human Isolates of Multidrug-Resistant Non-typhoidal Salmonella enterica

Front Microbiol. 2020 Dec 22:11:592223. doi: 10.3389/fmicb.2020.592223. eCollection 2020.

Authors

Affiliations

¹ Institute of Molecular Biology, National Academy of Sciences of RA, Yerevan, Armenia.
² Department of Clinical Laboratory Diagnostics, Yerevan State Medical University after M. Heratsi, Yerevan, Armenia.
³ Nork Infectious Clinical Hospital, Ministry of Health of RA, Yerevan, Armenia.
⁴ George Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilisi, Georgia.
⁵ Department of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia.
⁶ Queen Astrid Military Hospital, Brussels, Belgium.
⁷ The School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, United Kingdom.
⁸ Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.

Abstract

A total of 291 non-duplicate isolates of non-typhoidal Salmonella (NTS) were collected from the fecal samples of patients with salmonellosis in Armenia and Georgia during 1996-2016. The isolates were tested for resistance to antimicrobials, including extended-spectrum β-lactamases (ESBL). The high prevalence of multidrug-resistance (MDR) and ESBL-producer phenotypes was detected among Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) isolates collected from patients in Armenia between 1996 and 2016. A total of 36 MDR NTS isolates were subjected to whole genome sequencing (WGS) to determine the genetic background of antimicrobial resistance (AMR) and mobile genetic elements. All ESBL-producing S. Typhimurium isolates belonged to the same sequence type (ST328). The ESBL-producer phenotype was associated with plasmid-encoded CTX-M-5 production. A range of other plasmids was associated with resistance to other antimicrobials, including the MDR phenotype.

Keywords: WGS; antimicrobial resistance; antimicrobial resistance genes; extended-spectrum β-lactamases; non-typhoidal Salmonella.